PMID: 2492046Feb 1, 1989Paper

Detection and partial characterization of a developmentally regulated nuclear antigen in neural cells in vitro and in vivo

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
K SchillingC Pilgrim

Abstract

We report that a monoclonal antibody directed against phosphorylated neurofilaments (SMI 31) recognizes nuclear antigens present in embryonic but not in adult neural cells. On Western blots, the antibody reacts with four proteins of apparent MW 35, 37, 52/54, and 250 KD which are found exclusively in developing brain tissue. These nuclear antigens are expressed by glial and neuronal cells. Both nuclear staining and immunoreactive proteins decrease with ongoing in vitro differentiation. A computer search for proteins that share the epitope recognized by antibody SMI 31 did not yield any proteins of known nuclear localization that exhibit the same molecular weights and solubility characteristics as the above immunoreactive proteins. We conclude that antibody SMI 31 recognizes hitherto unknown nuclear proteins which, in neural cells, are developmentally regulated.

Citations

Dec 15, 1993·Journal of Neuroscience Research·L M NotterpekL H Rome
Dec 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D DahlV H Gilad
Aug 1, 1994·Neuron·L M Notterpek, L H Rome
Nov 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Parvathi RudrabhatlaHarish C Pant
Jul 25, 2009·Muscle & Nerve·Mohammad SalajeghehSteven A Greenberg
Mar 17, 2004·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Jean-Pierre de ChadarévianChristos D Katsetos
Apr 9, 2002·Brain Research·Tracy L GlassJoseph R Koke

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