Dec 17, 1975

Detection and partial characterization of lipoprotein lipase in bovine aorta

Biochimica Et Biophysica Acta
L C Henson, M C Schotz

Abstract

Extracts of acetone-ether powders of bovine thoracic aorta contain lipase activity which has an alkaline pH maximum (7.8-8.4) and is stimulated 4-10-fold by adding serum or isolated apolipoprotein-glutamate to the assay mixture. Serum activation is completely reversed by isolated apolipoprotein-serine or apolipoprotein-alanine. Lipolysis is strongly inhibited by NaCl (0.5 M) and protamine sulfate (1 mg/ml) and partially inhibited by heparin. Based on these characteristics, the lipase is identified as lipoprotein lipase.

  • References10
  • Citations9

Mentioned in this Paper

Protamine Sulfate (USP)
Liquaemin
Blood
PRM1
Lipoproteins
Lipoprotein LIPASE
Sodium Chloride, (24)NaCl
Bos indicus
Descending Aorta
Hydrogen-Ion Concentration

About this Paper

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.