Detection and Partial Molecular Characterization of Two Plum pox virus Isolates from Plum and Wild Apricot in Southeast Kazakhstan

Plant Disease
S SpiegelDelano James

Abstract

Plum pox virus (PPV) was detected in wild apricot and cultivated plum maintained in a germ plasm collection in Kazakhstan. Both isolates were typed as D strain, with no evidence of recombination. The virus was detected by triple-antibody sandwich enzyme-linked immunosorbent assay (ELISA) utilizing the universal PPV-specific monoclonal antibody (MAb) 5B as the secondary antibody, and by reverse-transcription polymerase chain reaction (RT-PCR) assay using primers that amplified a 243-bp fragment in the C-terminus of the coat protein (CP) coding region. Immunocapture (IC) RT-PCR was used to detect PPV in nine wild apricot accessions, including eight ELISA-negative and one ELISA-positive. The plum and apricot isolates reacted positively in Western blot assay with the universal MAb 5B, and negatively with the strain-M-specific MAb-AL. Restriction fragment length polymorphism analysis applied to the amplified 243-bp fragment showed that restriction sites for AluI and RsaI were present in the were present in the plum and apricot samples. An amplified 836-bp cDNA fragment derived from the P3-6K1 coding region of both isolates had restriction profiles typical for strain D. Nucleotide identities of 99 to 100% were observed for the 243-bp...Continue Reading

References

Aug 1, 1991·Journal of Virological Methods·T WetzelJ Dunez
Dec 11, 1989·Nucleic Acids Research·P Y TeycheneyJ Dunez
Jan 1, 2001·Plant Disease·D ThompsonD James

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Citations

Dec 4, 2012·Viruses·Jiri SochorRene Kizek
Sep 12, 2015·The New Phytologist·Stéphanie MarietteVéronique Decroocq
Dec 17, 2008·Archives of Virology·Erzsébet SzathmáryLászló Palkovics

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