PMID: 9002882Aug 1, 1996Paper

Detection and prevalence of the tetracycline resistance determinant Tet Q in the microbiota associated with adult periodontitis

Oral Microbiology and Immunology
J M Lacroix, C B Walker

Abstract

Subgingival plaque samples were collected from 68 patients with a history of moderate to severe adult periodontitis and enumerated on Trypticase-soy blood agar plates, with and without tetracycline at 4 micrograms/ml. Each different colony morphotype was enumerated, and a representative colony was subcultured for identification and examined for the tetracycline resistance gene tet(Q) by polymerase chain reaction (PCR) amplification and DNA hybridization, using a fragment of tetA(Q)2 from Bacteroides fragilis 1126. PCR primers (5'-GGCTTCTACGACATCTATTA-3' and 5'-CATCAACATTTATCTCTCTG-3') were chosen to amplify a 755 bp region of tet(Q). The subgingival plaque samples were also tested by PCR. Approximately 12% of the total cultivable flora was resistant to tetracycline, and the percentage of the tetracycline-resistant cultivable flora with the tet(Q) gene varied greatly from one patient to another with a range from 0.0 to 67%. Half of the 68 subgingival plaque samples were positive or weakly positive for tet(Q) by PCR. Approximately 15% of the 210 isolates subcultured with resistance to tetracycline, (> or = 4 micrograms/ml) contained tet(Q), and 60% contained tet(M). All of the tet(Q)-resistant isolates were gram-negative anaerobi...Continue Reading

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Citations

Sep 12, 1998·Otolaryngologic Clinics of North America·S M KerpelP D Freedman
Oct 28, 2009·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Robert T JetersAbigail A Salyers
Sep 5, 2009·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Lorna A SevilleAdam P Roberts
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·A VilledieuP Mullany
Oct 5, 2001·Clinical Microbiology Reviews·A C FluitF J Schmitz
Aug 4, 2012·Journal of Applied Oral Science : Revista FOB·Geisla Mary Silva SoaresMagda Feres
Apr 29, 2005·Journal of Periodontology·Sajith AbrahamN D Jayakumar
Apr 3, 2012·Journal of Oral Science·Mohammadmehdi Fani, Jamshid Kohanteb
Jul 26, 2014·Journal of Periodontology·Lorenzo DragoSilvio Taschieri
Dec 20, 2011·Veterinary Microbiology·María LorenzoAlberto Quesada
Sep 10, 2010·Anaerobe·Gena D TribbleCharles J Smith
Jun 23, 2009·Journal of Clinical Periodontology·Ioannis IoannidisAntonis Konstantinidis
May 15, 2004·Journal of Clinical Periodontology·Rosa Maria J RodriguesAna Paula V Colombo
Dec 16, 2004·Archives of Oral Biology·W Keung LeungEsmonde F Corbet
Aug 5, 2006·Anaerobe·J C Fenno, B C McBride
Dec 4, 2014·International Journal of Antimicrobial Agents·Clarisse DupinAnne Jolivet-Gougeon
Sep 7, 1999·Journal of Periodontology·I M MadinierL R Hannoun
Aug 28, 2004·Periodontology 2000·Makoto UmedaIsao Ishikawa
Feb 7, 2021·Applied and Environmental Microbiology·Paiboon TunsagoolGunnaporn Suriyaphol

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