PMID: 2502536Aug 1, 1989Paper

Detection and properties of A-factor-binding protein from Streptomyces griseus

Journal of Bacteriology
K MiyakeT Beppu

Abstract

The optically active form of tritium-labeled A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone), a pleiotropic autoregulator responsible for streptomycin production, streptomycin resistance, and sporulation in Streptomyces griseus, was chemically synthesized. By using the radioactive A-factor, a binding protein for A-factor was detected in the cytoplasmic fraction of this organism. The binding protein had an apparent molecular weight of approximately 26,000, as determined by gel filtration. Scatchard analysis suggested that A-factor bound the protein in the molar ratio of 1:1 with a binding constant, Kd, of 0.7 nM. The number of the binding protein was roughly estimated to be 37 per genome. The "inducing material" virginiae butanolide C (VB-C), which has a structure very similar to that of A-factor and is essential for virginiamycin production in Streptomyces virginiae, did not inhibit binding. In addition, no protein capable of specifically binding 3H-labeled VB-C was found in S. griseus. Together with the observation that VB-C had almost no biological activity on the restoration of streptomycin production or sporulation in an A-factor-deficient mutant of S. griseus, these results indicated that the binding protein...Continue Reading

References

Sep 1, 1982·The Journal of Antibiotics·O Hara, T Beppu
Dec 1, 1982·The Journal of Antibiotics·U GräfeL Radics

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Citations

Jul 15, 2009·Biotechnology and Applied Biochemistry·Sheetal RainaTajalli Keshavarz
Jan 1, 1990·Critical Reviews in Biotechnology·S Horinouchi, T Beppu
Jan 1, 1993·Antonie van Leeuwenhoek·S Horinouchi, T Beppu
Jun 6, 2003·Bioscience, Biotechnology, and Biochemistry·Makoto HashimotoMasahiro Natsume
Jul 2, 2009·Annual Review of Microbiology·Paul D Straight, Roberto Kolter
Jan 17, 2012·Applied Microbiology and Biotechnology·Yung-Hun YangByung-Gee Kim
Feb 1, 1995·Journal of Bacteriology·M RuengjitchatchawalyaY Yamada
Nov 1, 1995·Journal of Bacteriology·H OnakaS Horinouchi
Nov 26, 2010·Chemical Reviews·Joanne M Willey, Alisa A Gaskell
Sep 8, 2021·Journal of Microbiology and Biotechnology·Jianxin DongWenjun Guan

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