Detection and quantification of house mouse Eimeria at the species level - Challenges and solutions for the assessment of coccidia in wildlife

International Journal for Parasitology. Parasites and Wildlife
V.H. Jarquín-DiazE Heitlinger

Abstract

Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, prevalence of coccidia is often not assessed at the parasite species level in non-livestock animals. Parasite species - specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse (Mus musculus) in Germany, and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate bias towards a particular parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each parasite species along the distal-proximal axis of the intestine. Parasite genotyping from tissue samples provides additional indication for the absence of...Continue Reading

Citations

Feb 23, 2020·Ecology and Evolution·Víctor Hugo Jarquín-DíazEmanuel Heitlinger
Jan 5, 2021·Ecology and Evolution·Alice BalardEmanuel Heitlinger
May 15, 2021·BMC Veterinary Research·Shengyong FengHongxuan He
Jun 6, 2021·International Journal for Parasitology·Aneta TrefancováVáclav Hypša

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Datasets Mentioned

BETA
KX495129
MH777539

Methods Mentioned

BETA
genotyping
PCRs
PCR
electron microscopy

Software Mentioned

R
Geneious
PhyML
Adobe Photoshop CC
R Development Core Team
epiR ”
JModelTest
MUSCLE
FigTree
BLAST

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