Detection and quantification of human and bovine noroviruses by a TaqMan RT-PCR assay with a control for inhibition

Molecular and Cellular Probes
A ScipioniE Thiry

Abstract

Noroviruses are single-stranded RNA viruses belonging to the family Caliciviridae. They are a major cause of epidemic and sporadic gastroenteritis in humans and calves. Reverse transcription-polymerase chain reaction (RT-PCR) has become the "gold standard" for detection of noroviruses in faecal and environmental samples. However, false negative results due to co-concentration of RT-PCR inhibitors are a continuous concern. A TaqMan real-time RT-PCR assay making use of a foreign internal RNA control and a RNA standard was developed. Very interestingly, this method is capable of detecting human noroviruses belonging to genogroups I and II, and bovine noroviruses belonging to genogroup III. Inhibitors were removed efficiently by 1/10 dilution of the sample or addition of bovine serum albumin to the RT-PCR mix. This assay was validated with human and bovine stool samples previously tested for norovirus by conventional RT-PCR. The ability to detect norovirus in stool samples that were negative by conventional RT-PCR assay demonstrate the higher sensitivity of the TaqMan assay compared to the conventional RT-PCR assay. This real-time RT-PCR assay allows the detection of both human and bovine noroviruses, avoids false negative results ...Continue Reading

References

Nov 1, 1978·Journal of Medical Microbiology·G N Woode, J C Bridger
Jul 1, 1993·Virology·X JiangM K Estes
Aug 4, 1998·Archives of Virology·M SugiedaS Nakajima
Oct 8, 1999·Emerging Infectious Diseases·P S MeadR V Tauxe
May 11, 2000·The Journal of Infectious Diseases·A Z Kapikian
Jan 9, 2001·Clinical Microbiology Reviews·R L Atmar, M K Estes
Jan 30, 2003·Veterinary Microbiology·Wim H M van der PoelMarion P G Koopmans
May 8, 2003·Journal of Clinical Microbiology·Steven B Kleiboeker
Jan 14, 2004·The Journal of General Virology·Erwin DuizerMary K Estes
Apr 7, 2004·Acta Clinica Belgica·V VerbelenP Goubau
Mar 7, 2006·Applied and Environmental Microbiology·Veronica CostantiniLinda J Saif
Apr 7, 2006·Journal of Clinical Microbiology·A Angelica TrujilloStephan S Monroe
Jun 8, 2007·Emerging Infectious Diseases·Timothy M StraubCheryl A Nickerson
Feb 26, 2008·The Veterinary Journal·A ScipioniE Thiry

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Citations

Feb 16, 2013·Food and Environmental Virology·Ambroos StalsMieke Uyttendaele
Aug 17, 2010·Applied and Environmental Microbiology·Maha ZakhourJacques Le Pendu
Jul 29, 2010·BMC Research Notes·Lydia V RumpNarjol Gonzalez-Escalona
Dec 4, 2015·The Veterinary Journal·Elisabetta Di FeliceEtienne Thiry
Feb 24, 2009·Veterinary Microbiology·Axel MauroyEtienne Thiry
Dec 3, 2014·Forensic Science International. Genetics·Tao Geng, Richard A Mathies
Dec 7, 2011·Journal of Biological Engineering·Felipe T Lee-MontielMark R Riley
Jan 9, 2015·Clinical Microbiology Reviews·Elizabeth RobilottiBenjamin A Pinsky
Jul 20, 2018·Journal of Clinical Microbiology·Jesse J WaggonerBenjamin A Pinsky
Jun 24, 2016·The Open Microbiology Journal·Haifeng Chen, Yuan Hu
Apr 25, 2012·Analytical and Bioanalytical Chemistry·E M Hartmann, R U Halden
Jul 4, 2012·Journal of Applied Microbiology·C SchraderR Johne
Sep 24, 2020·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·K M GuarinesL J Pena
Aug 21, 2021·Journal of Environmental Sciences (China)·Alex H S ChikUNKNOWN Canadian SARS-CoV-2 Inter-Laboratory Consortium

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