Detection and quantitation of human immunodeficiency virus type-1 particles by confocal microscopy

Journal of Virological Methods
Bishnupriya BhattacharyaAriberto Fassati

Abstract

A method is described to visualise directly human immunodeficiency virus type-1 (HIV-1) particles. HIV-1 containing samples were adsorbed onto a plastic surface and doubly labeled with antibodies specific for viral proteins and sensitive nucleic acids dyes. Laser scanning confocal microscopy detected co-localization of viral proteins and nucleic acids, thus allowing specific identification of HIV. Using this technique, we have quantified eight different HIV-1 sub-types and three HIV-1 groups in tissue culture supernatants from infected peripheral blood mononuclear cells (PBMCs). Confocal counts correlated well with electron microscopy (EM) counts and HIV-1 RNA loads as determined by quantitative PCR. Confocal microscopy may prove to be a simple alternative to electron microscopy for virus identification and quantitation.

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Citations

Jun 19, 2012·AIDS·Thumbi Ndung'u, Robin A Weiss
Mar 27, 2009·Journal of Clinical Microbiology·Delphine DesboisElisabeth Dussaix
Jun 19, 2015·AIDS Research and Human Retroviruses·Marina R AlexanderPer Johan Klasse
Jun 15, 2006·Analytical Chemistry·Marian NavratilEdgar A Arriaga

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