Detection and quantitation of Staphylococcus aureus penicillinase plasmid deoxyribonucleic acid by reassociation kinetics.

Journal of Bacteriology
M RushR DeLap

Abstract

The quantity of penicillinase plasmid deoxyribonucleic acid (DNA) in various strains of Staphylococcus aureus has been determined by DNA-DNA reassociation kinetics. Specifically, 32P- or 125I-labeled denatured probes of purified plasmid DNA were reassociated in the presence of denatured DNAs isolated from the bacterial strains in question. The number of plasmid copies per cell was calculated from the effect of the latter nucleic acid samples on the reassociation rate of the radiolabeled probe. Among the S. aureus strains examined were monoplasmid, diplasmid and replication-defective representatives, and the effect of temperature on wild-type plasmid content was also investigated.

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Citations

Feb 1, 1980·Molecular & General Genetics : MGG·M R OttenR Seelke
Mar 5, 2004·Journal of Bacteriology·Ching Wen TsengGeorge C Stewart
Jul 1, 1995·Bioorganic & Medicinal Chemistry·J Light, R A Lerner
May 1, 1986·Applied and Environmental Microbiology·L S HeathH E Heath

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