Mar 1, 1976

Detection and quantitative estimation of morphine in the urine of drug addicts by combined 2-dimensional thin-layer chromatography and spectrofluorometry

European Journal of Toxicology and Environmental Hygiene. Journal Européen De Toxicologie
A Viala, M Estadieu


Following extraction from urine and thin-layer bidimensional chromatography, the suspected spot of morphine is located by UV examination at 350 nm and eluted with methanol by means of "Eluchrom" apparatus. The dried residue is then subjected to spectrofluorimetric analysis, in definite conditions. This procedure can be used to confirm the identification of the alkaloid and to achieve its estimation. The sensitivity and the recoveries of various quantities of morphine added to urine were determined.

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Mentioned in this Paper

Plant alkaloid
Morphine Abuse
Morphine Measurement
Fluorescence Spectroscopy
Morphine Sulfate (2: 1), Pentahydrate
Urine - SpecimenType

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