Detection and serotype-specific differentiation of vesicular stomatitis virus using a multiplex, real-time, reverse transcription-polymerase chain reaction assay

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Kate HoleLuis A Pineda

Abstract

A multiplex, real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that allowed simultaneous detection and rapid differentiation of vesicular stomatitis virus strains--New Jersey (VSV-NJ) and Indiana 1, 2, and 3 (VSV-IN1-3). This assay involves use of a set of VSV universal primers located in the L gene that amplify VSV-IN1-3 and VSV-NJ using probes that allow differentiation of the major serotypes Indiana and New Jersey. The assay was evaluated using reference VSV, foot-and-mouth disease virus, swine vesicular disease virus, and vesicular exanthema of swine virus. To estimate diagnostic sensitivity, 159 epithelial samples collected between 1996 and 2002 from naturally infected cattle in Colombia were used. The assay cut off was calculated by testing RNA extracted from 150 virus-negative bovine tissues consisting of tongue, soft palate, muzzle, coronary band, and lymph node. All infected cattle were test positive for VS by results of real-time RT-PCR analysis; results for 156 of 159 (98.1%) agreed with the serotype determination from the complement-fixation test. Amplification did not occur in any of the negative bovine epithelial samples, allowing the cut-off values for the assay to be set. The...Continue Reading

References

Mar 1, 1990·The American Journal of Tropical Medicine and Hygiene·L L RodriguezG J Letchworth
Nov 1, 1985·American Journal of Epidemiology·D E StallknechtG A Erickson
Dec 1, 1994·Journal of Virological Methods·M C HöfnerF Ariza Botero
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·L L RodríguezS T Nichol
Aug 7, 1998·Journal of Virological Methods·J I NúñezF Sobrino
Sep 19, 2003·The Journal of General Virology·Elizabeth R ChareEdward C Holmes
Dec 12, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Roberta J MagnusonMo Salman

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Citations

Jan 6, 2012·Journal of Biomedicine & Biotechnology·Nicholas JohnsonAnthony R Fooks
May 31, 2011·Journal of Virological Methods·Oliver LungDirk Deregt
Jun 9, 2016·Memórias do Instituto Oswaldo Cruz·Aline Lavado TolardoLuiz Tadeu Moraes Figueiredo
May 11, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kate HoleAlfonso Clavijo
Dec 30, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steve P CramptonSilvia Bolland
Jul 7, 2018·Clinical Microbiology Reviews·Naveen KumarBarry T Rouse
Oct 24, 2019·ELife·Rachel A LiberatorePaul D Bieniasz
May 26, 2020·Archives of Virology·Mariana Lázaro SalesJenner Karlisson Pimenta Dos Reis

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Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Kate HoleA Clavijo
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