Detection and Specific Enumeration of Multi-Strain Probiotics in the Lumen Contents and Mucus Layers of the Rat Intestine After Oral Administration

Probiotics and Antimicrobial Proteins
Hee Ji LeeJ Paul Fawcett

Abstract

Although the detection of viable probiotic bacteria following their ingestion and passage through the gastrointestinal tract (GIT) has been well documented, their mucosal attachment in vivo is more difficult to assess. In this study, we investigated the survival and mucosal attachment of multi-strain probiotics transiting the rat GIT. Rats were administered a commercial mixture of the intestinal probiotics Lactobacillus acidophilus LA742, Lactobacillus rhamnosus L2H and Bifidobacterium lactis HN019 and the oral probiotic Streptococcus salivarius K12 every 12 h for 3 days. Intestinal contents, mucus and faeces were tested 6 h, 3 days and 7 days after the last dose by strain-specific enumeration on selective media and by denaturing gradient gel electrophoresis. At 6 h, viable cells and DNA corresponding to all four probiotics were detected in the faeces and in both the lumen contents and mucus layers of the ileum and colon. Viable probiotic cells of B. lactis and L. rhamnosus were detected for 7 days and L. acidophilus for 3 days after the last dose. B. lactis and L. rhamnosus persisted in the ileal mucus and colon contents, whereas the retention of L. acidophilus appeared to be relatively higher in colonic mucus. No viable cells...Continue Reading

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Citations

Apr 16, 2011·Biological Trace Element Research·Woravimol KrittapholJ Paul Fawcett
Feb 22, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Hee Ji LeeJ Paul Fawcett
Nov 22, 2011·Applied and Environmental Microbiology·Philippe UlsemerSteffen Goletz
Mar 6, 2021·PloS One·Paul Mathias JansenGeorg Conrads

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