Detection and typing of low-risk human papillomavirus genotypes HPV 6, HPV 11, HPV 42, HPV 43 and HPV 44 by polymerase chain reaction and restriction fragment length polymorphism

Journal of Virological Methods
Polona J MaverBostjan J Kocjan

Abstract

A novel PCR-restriction fragment length polymorphism assay (PCR-RFLP) was developed for sensitive detection and reliable differentiation of five low-risk human papillomavirus (lr-HPV) genotypes: HPV 6, HPV 11, HPV 42, HPV 43 and HPV 44, as well as differentiation of prototypic and non-prototypic HPV 6 genomic variants. The assay is based on the amplification of a 320-bp fragment of the HPV E1 gene and subsequent analysis of PCR-products with BsaJI and HinFI. Testing on plasmid standards showed that PCR-RFLP enabled simple and reliable identification and differentiation of five targeted lr-HPV genotypes and could detect reproducibly down to 10 copies of viral genome equivalents per PCR. The PCR-RFLP showed almost complete agreement with previously obtained genotyping results on 42 HPV-DNA negative samples and 223 HPV-DNA positive samples (45 HPV 6, 34 HPV 11, 35 HPV 42, 10 HPV 43, 24 HPV 44 positive samples and 75 samples containing 28 non-targeted HPV genotypes). The novel assay is simple and robust, does not require any sophisticated equipment and can be of great value for epidemiological studies, particularly in settings in which financial resources are limited.

References

Jan 1, 1994·Virchows Archiv : an International Journal of Pathology·N GaleJ Fischinger
Feb 7, 2003·The New England Journal of Medicine·Nubia MuñozUNKNOWN International Agency for Research on Cancer Multicenter Cervical Cancer Study Group
Jun 9, 2004·Virology·Ethel-Michele de VilliersHarald zur Hausen
May 17, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Gary M CliffordJeanne M Pimenta
May 15, 2007·The New England Journal of Medicine·Suzanne M GarlandUNKNOWN Females United to Unilaterally Reduce Endo/Ectocervical Disease (FUTURE) I Investigators
Jul 22, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·François AubinUNKNOWN EDiTH Study Group
Aug 30, 2008·Journal of Virological Methods·Bostjan J KocjanMario Poljak
Jun 6, 2009·Journal of Clinical Microbiology·Mario PoljakAdriana Vince
Jul 15, 2009·Virology·Bostjan J KocjanKatja Seme
Mar 9, 2010·Virology·Hans-Ulrich BernardEthel-Michele de Villiers

❮ Previous
Next ❯

Citations

Nov 8, 2012·Virology Journal·André L P AbreuMarcia E L Consolaro
Mar 12, 2013·Journal of Virological Methods·Catharina E CombrinckFelicity J Burt
Oct 31, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Boštjan J KocjanMario Poljak
Jul 3, 2013·Public Health Nursing·Katharine J HeadLaurel A Mills
Jun 26, 2014·International Journal of Dermatology·Hrishikesh Kumar BelaniKim Bohjanen
Jul 19, 2014·Bulletin of Mathematical Biology·Aliya A Alsaleh, Abba B Gumel
Feb 7, 2017·Expert Reviews in Molecular Medicine·D TsakogiannisP Markoulatos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Bostjan J KocjanMario Poljak
Virology
Ethel-Michele de VilliersHarald zur Hausen
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Anco MolijnLeen-Jan van Doorn
APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
Vincent R BonaguraAllan L Abramson
© 2021 Meta ULC. All rights reserved