Detection and typing of molluscum contagiosum virus in skin lesions by using a simple lysis method and polymerase chain reaction

Journal of Medical Virology
A NuñezA Martin-Gallardo

Abstract

A polymerase chain reaction (PCR) assay for the rapid detection and typing of molluscum contagiosum virus (MCV) was developed. The target DNA was a 393 base pair (bp) segment, which is present in the coding region of the MCV p43K gene product. Release of MCV DNA from skin lesions was performed by using a simple procedure that provided suitable template DNA for amplification, and allowed detection of MCV directly in clinical material. The PCR yielded a unique 393 bp product when MCV DNA was used as template. This product was not shown with DNA from other viruses and bacterial pathogens causing skin diseases. The specific PCR product was obtained with individual lesions from all patients clinically diagnosed with MCV infection, whereas no products were detected with skin samples from healthy individuals. Sequencing of this PCR product allowed determination of the virus subtype on the basis of previously described nucleotide differences between subtypes MCVI and MCVII. To avoid the sequencing process, a second PCR assay was developed, in which the target DNA sequence included a MCVI-specific recognition site for the restriction endonuclease BamHI. This PCR assay yielded a unique 575 bp product with lesions from either MCVI- or MCV...Continue Reading

References

Sep 1, 1992·Journal of Medical Virology·C D Porter, L C Archard
May 1, 1992·The British Journal of Dermatology·R J KoopmanW M Dolmans
Oct 1, 1990·Journal of Clinical Microbiology·U SjöbringH Miörner
Sep 1, 1990·Journal of Medical Virology·C H ThompsonI M Biggs
Jan 1, 1988·Nucleic Acids Research·E DamsR De Wachter
Oct 1, 1987·Epidemiology and Infection·C D PorterL C Archard
Oct 1, 1988·The Journal of Infectious Diseases·J ScholzR Postlethwaite
Jul 1, 1985·Archives of Dermatology·P C Lombardo
Sep 1, 1970·Archives of Environmental Health·R Postlethwaite
Jul 1, 1981·Sexually Transmitted Diseases·S T BrownS J Kraus
Nov 10, 1995·Virology·R M BullerJ Kreider
Oct 1, 1993·Virology·G P JohnsonE Paoletti

❮ Previous
Next ❯

Citations

Aug 27, 2013·The Lancet Infectious Diseases·Xiaoying ChenJoachim J Bugert
Nov 13, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jason P TramaEli Mordechai
Apr 18, 2000·The Journal of General Virology·V P SmithA Alcamí
Dec 2, 2004·Dermatologic Therapy·Arun Chakrabarty, Karl Beutner
Jan 10, 2001·Journal of the American Academy of Dermatology·D G Diven

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.