Apr 3, 2018

Detection and validation of EST-SSR markers associated with sugar-related traits in sugarcane using linkage and association mapping

Genomics
Kittipat UkoskitSomvong Tragoonrung

Abstract

Sugar-related traits are of great importance in sugarcane breeding. In the present study, quantitative trait loci (QTL) mapping validated with association mapping was used to identify expressed sequence tag-simple sequence repeats (EST-SSRs) associated with sugar-related traits. For linkage mapping, 524 EST-SSRs, 241 Amplified Fragment Length Polymorphisms, and 10 genomic SSR markers were mapped using 283 F1 progenies derived from an interspecific cross. Six regions were identified using Multiple QTL Mapping, and 14 unlinked markers using single marker analysis. Association analysis was performed on a set of 200 accessions, based on the mixed linear model. Validation of the EST-SSR markers using association mapping within the target QTL genomic regions identified two EST-SSR markers showing a putative relationship with uridine diphosphate (UDP) glycosyltransferase, and beta-amylase, which are associated with pol and sugar yield. These functional markers can be used for marker-assisted selection of sugarcane.

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Mentioned in this Paper

Biological Markers
Gene Polymorphism
Study
Short Tandem Repeat
Quantitative Trait Loci
SSR1
Genome
Uridine Diphosphate
Plant Breeding
Molecular Probe Techniques

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