Detection by competitive enzyme-linked immunosorbent assay of a bovine serum albumin peptide (ABBOS) in infant formulas based on hydrolyzed cow's milk protein

Diabetes Care
E C van Beresteijn, R J Meijer

Abstract

Our aim was to determine whether currently available products based on hydrolyzed milk protein and a small peptide (ABBOS) in bovine serum albumin (BSA) (positions 126-144) share common antigenic sites. The commercial products are primarily developed to reduce cow's milk protein-associated allergenicity, but whether they also could be used in intervention trials to elucidate the role of BSA in the etiology of IDDM is not known. A sensitive competitive enzyme-linked immunosorbent assay (ELISA) using a rabbit polyclonal antibody raised against the ABBOS peptide in BSA was developed. With this method, we determined cross-reactivity between the ABBOS peptide and commercially available products: four milk protein hydrolysates and eight infant formulas based on hydrolyzed milk protein. The products were further characterized by physicochemical techniques. Hydrolyzed milk protein products are found to inhibit competitively the binding of ABBOS peptide to antibody. The number of residual reactive sites varied considerably among products and was not strongly related to the degree of hydrolysis (DH) or the molecular mass distribution of the hydrolysates. The presence of possible immunoreactive peptides in infant formulas based on hydroly...Continue Reading

Citations

Jan 24, 2007·The Journal of Allergy and Clinical Immunology·Andrea von BergUNKNOWN German Infant Nutritional Intervention Study Group
Dec 28, 1999·Journal of the American Dietetic Association·P Z MarincicD G Hendricks
Oct 26, 2000·Journal of Dairy Science·A Rosendal, V Barkholt

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