Detection by ELISA of C-terminal proBNP in plasma from cats with cardiomyopathy

The Veterinary Journal
Philip F SolterD David Sisson

Abstract

The B-type natriuretic peptide prohormone (proBNP) is enzymatically cleaved into an inactive N-terminal peptide and a biologically active C-terminal peptide with many beneficial cardiorenal effects. The purpose of this study was to develop and test in cats with cardiomyopathy an immunoassay to quantify the concentrations of C-terminal proBNP in feline plasma. An anti-canine proBNP monoclonal antibody (UI-1021) was shown to have adequate binding affinity to proBNP 80-106 for use in a solid-phase immunoassay, and by epitope mapping to bind within positions 84-87 of feline proBNP. UI-1021 was paired with an affinity-purified rabbit polyclonal detection antibody to feline proBNP 100-106, in a sandwich ELISA with feline proBNP 80-106 standard. The linearity and analytical range and sensitivity of the assay were confirmed from 1.4 to 85 pmol/L. Spike recovery averaged 106.5% (95% confidence interval 78-135%). Within run and intra-assay coefficients of variation were <12%. A protease inhibitor mixture preserved proBNP 80-106 immunoreactivity for at least 5 days in plasma. Clinical verification of the ELISA was done using plasma from 13 cats with cardiomyopathy, whose C-terminal proBNP concentrations ranged from 1.7 to 78.8 pmol/L vs. ...Continue Reading

References

Jun 26, 1987·Journal of Immunological Methods·J D BeattyW G Vlahos
Feb 23, 2002·American Journal of Veterinary Research·Alexander W BiondoPhilip E Solter
Jul 18, 2002·Gene·Zonglin L LiuPhilip F Solter
Nov 18, 2005·Cardiovascular Research·Toshio NishikimiHiroaki Matsuoka
Nov 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Adam M HawkridgeDavid C Muddiman
Mar 14, 2007·Journal of the American College of Cardiology·Faquan LiangAndrew A Protter
Mar 14, 2007·Journal of the American College of Cardiology·Horng H Chen
Mar 21, 2007·Journal of the American College of Cardiology·Carolyn S P LamMargaret M Redfield
Feb 22, 2008·Journal of Veterinary Internal Medicine·D J ConnollyM Metcalf
Mar 28, 2008·Veterinary Clinical Pathology·Philip F SolterD David Sisson
May 10, 2008·Journal of the American College of Cardiology·Stephen W WaldoAlan S Maisel
Apr 28, 2009·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·David J ConnollyHarriet M Syme
Apr 28, 2009·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·Philip R FoxDaniel F Hogan
Apr 29, 2009·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·Stephanie M LalorHarriet M Syme
Dec 17, 2009·Clinical Cardiology·Santosh G MenonAndrew A Protter
Jul 9, 2010·The Veterinary Clinics of North America. Small Animal Practice·David J Connolly
Feb 23, 2011·Current Heart Failure Reports·Toshio NishikimiKazuwa Nakao
Nov 3, 2011·European Journal of Heart Failure·Nelson GomezKathleen Mc Entee
Apr 23, 2013·Journal of the American College of Cardiology·Jeffrey B GeskePaul Sorajja
Mar 14, 2014·JACC. Heart Failure·Lisa C Costello-BoerrigterJohn C Burnett
Oct 22, 2014·Current Heart Failure Reports·Paul M McKie, John C Burnett
Oct 24, 2014·Circulation. Heart Failure·Brenda K HuntleyTomoko Ichiki

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