Detection by PCR of adhesins genes and slime production in clinical Staphylococcus aureus

Journal of Basic Microbiology
Tarek ZmantarAmina Bakhrouf

Abstract

The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus strains were considered important factors of virulence. In this study, 46 strains of Staphylococcus aureus were isolated from auricular infection, and were investigated for slime production using Congo Red Agar method (CRA). In order to detect the adhesins genes (ica A, ica D, fnb A, cna, Clf A) Polymerase Chain Reaction was used. Qualitative biofilm production of S. aureus using CRA plates revealed that 56.5% of strains were slime producers. In addition 78.26% of strains were ica A and ica D positive. While the fnbA gene was present in 76.1% of isolated strains. Furthermore, 56.5% of strains have the cna gene and 30.4% were clfA positives. Overall this study confirms the presence of fnb A and ica A/ica D genes in the majority of studies S. aureus strains isolated from Staphylococcal sepsis.

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Citations

Nov 19, 2011·Tropical Animal Health and Production·Somanath Kundalik ChavhanNitin Vasantrao Kurkure
May 29, 2013·Journal of Basic Microbiology·J M Liesse IyambaJ P Dehaye
Jun 10, 2015·Microbial Pathogenesis·Abderrahmen MerghniMaha Mastouri
Jun 23, 2015·Nigerian Journal of Clinical Practice·A DundarA Sengun
May 29, 2013·Microbial Pathogenesis·Zulfiqar Ali MiraniSeema Ismat Khan

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