Detection by PCR of pathogenic protozoa in raw and drinkable water samples in Colombia

Parasitology Research
Jessica Triviño-ValenciaJorge Enrique Gómez-Marín

Abstract

We evaluated the presence of DNA of Giardia, Toxoplasma, and Cryptosporidium by PCR, and of Giardia and Cryptosporidium genera by immunofluorescence antibody test (IFAT), in water samples, before, during, and after plant treatment for drinkable water. We applied this method in 38 samples of 10 l of water taken from each of the water treatment steps and in 8 samples taken at home (only for Toxoplasma PCR) in Quindio region in Colombia. There were 8 positive samples for Cryptosporidium parvum (21 %), 4 for Cryptosporidium hominis (10.5 %), 27 for Toxoplasma gondii (58.6 %), 2 for Giardia duodenalis assemblage A (5.2 %), and 5 for G. duodenalis assemblage B (13.1 %). By IFAT, 23 % were positive for Giardia and 21 % for Cryptosporidium. An almost perfect agreement was found between IFAT and combined results of PCR, by Kappa composite proportion analysis. PCR positive samples were significantly more frequent in untreated raw water for C. parvum (p = 0.02). High mean of fecal coliforms, high pH values, and low mean of chlorine residuals were strongly correlated with PCR positivity for G. duodenalis assemblage B. High pH value was correlated with PCR positivity for C. parvum. Phylogenetic analysis of DNA sequences was possible, showin...Continue Reading

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Citations

May 25, 2016·Water Research·Fabiana Lora-SuarezJorge E Gomez-Marin
Jul 9, 2016·PloS One·Beatriz Coronato NunesFilipe A Carvalho-Costa
Feb 28, 2018·Parasitology Research·Catalina AvendañoJoaquín Quílez
Oct 30, 2019·Parasitology Research·Julio César LunaJorge Enrique Gómez-Marín
Apr 18, 2020·Parasite : Journal De La Société Française De Parasitologie·Alejandro Zamora-VélezJorge Enrique Gómez-Marín
Apr 18, 2017·Foodborne Pathogens and Disease·Ivonne B Hernandez-CortazarMatilde Jimenez-Coello
Dec 5, 2019·Frontiers in Cellular and Infection Microbiology·Monica Vargas-MontesJorge Enrique Gómez-Marín
May 22, 2020·Acta Parasitologica·Alejandro Zamora-VélezJorge Enrique Gómez-Marín
Nov 30, 2020·Zoonoses and Public Health·Luis Javier Pérez-GrisalesJuan Fernando Díaz-Nieto
Feb 26, 2020·Food and Waterborne Parasitology·Karen ShapiroIsabelle Villena
Apr 27, 2021·Heliyon·Jorge Enrique Gómez-MarínAlejandra de-la-Torre
Jun 11, 2021·Biomédica : revista del Instituto Nacional de Salud·Diana Marcela Campo-PortacioPedro José Blanco-Tuirán

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