Detection by polymerase chain reaction of genes encoding aminoglycoside-modifying enzymes in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types. Belgian Study Group of Hospital Infections (GDEPIH/GOSPIZ)

Journal of Medical Microbiology
R VanhoofE Hannecart-Pokorni

Abstract

The polymerase chain reaction (PCR) was used to identify the aacA-aphD, aphA3 and aadC genes, encoding the aminoglycoside-modifying enzymes AAC(6')-APH(2"), APH(3')III and ANT(4'4"), respectively, and the methicillin resistance determinant mecA, in epidemic aminoglycoside and methicillin-resistant isolates of Staphylococcus aureus. In total, 37 isolates collected in the period 1980-1985 and 81 isolates from the period 1991-1992 were obtained from 10 different Belgian hospitals. Epidemic isolates from the earlier period were characterised by phage type C (6/47/54/75) of phage group III, whereas two other epidemic phage types of group III-types A (77) and B (47/54/75/77/84/85)--were commonest in isolates from the second period. The bifunctional AAC(6')-APH(2") was the enzyme encountered most frequently. The prevalence of APH(3')III decreased significantly in the 1991-1992 period, while ANT(4',4") was found solely in isolates from this period. Resistance mechanisms were more complex in isolates from the 1991-1992 period and the mecA gene was detected in all isolates. The PCR results corresponded well with those obtained in the radiochemical phosphocellulose paper binding assay. Isolates from the 1991-1992 period were shown to expr...Continue Reading

Citations

Jan 23, 2013·Medical Molecular Morphology·Kenji FukutsujiTamotsu Harada
Mar 3, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S VandendriesscheO Denis
Dec 13, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J BraunerO Denis
Jan 9, 1999·Burns : Journal of the International Society for Burn Injuries·M Prasanna, C Thomas
Jul 29, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J Van EldereH Bobbaers
Sep 24, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·O MelterH de Lencastre
Jul 27, 2007·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Tomasz HauschildStefan Schwarz
May 13, 2009·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohammad EmaneiniNazli Sotoudeh
Jun 6, 2009·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Abbas YadegarGholam Reza Goudarzi
Oct 5, 2001·Clinical Microbiology Reviews·A C FluitF J Schmitz
Nov 8, 2003·Journal of Clinical Microbiology·O MelterH de Lencastre
Jun 4, 2008·Folia Histochemica Et Cytobiologica·Tomasz HauschildElzbieta Tryniszewska
Jul 25, 2009·Emerging Infectious Diseases·Olivier DenisMarc J Struelens
Nov 15, 2011·Acta Veterinaria Hungarica·Boris HabrunZeljko Cvetnić
Nov 9, 2004·Folia Microbiologica·J StepánJ Doskar
Sep 12, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C WildemauweM Struelens
Jul 24, 2004·Journal of Medical Microbiology·Letícia A M CarneiroVânia L C Merquior
Mar 21, 2007·Veterinary Microbiology·A J de NeelingX W Huijsdens

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