Detection, isolation and enumeration of Yersinia enterocolitica from raw pork

International Journal of Food Microbiology
J Andrew HudsonS Monson

Abstract

The methods available for the isolation of Yersinia enterocolitica from foods are generally considered to be less than optimal, and methods for estimation of numbers are lacking. Such methods are needed to understand better the significance of foodborne yersiniosis and to provide data for exposure assessment. We describe a method for the detection and enumeration of Y. enterocolitica containing the pYV virulence plasmid (YeP+) in samples from pork surfaces. The method uses a multiplex PCR targeting the ail and virF genes to detect Y. enterocolitica after incubation of surface swabs in Yersinia enrichment broth according to Ossmer. Enumeration was achieved by adapting the enrichment to a most probable number (MPN) method format. A presumptive result was available within 24 h of sample receipt, and YeP+ isolates were confirmed within four days. The presence/absence and MPN methods were evaluated in a pilot survey of 34 packs of raw pork meat purchased from retail outlets in Christchurch, New Zealand. YeP+ was detected by PCR on meat from 32% of the packs, and YeP+ isolates were obtained from 18% of the samples. YeP+ were present at numbers ranging from 0.30 to 5.42 MPN/cm(2). This improved method for the detection and enumeration...Continue Reading

References

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Citations

Apr 10, 2012·Archivum Immunologiae Et Therapiae Experimentalis·Nataniel BiałasMikael Skurnik
Mar 28, 2012·Journal of Microbiological Methods·Aruni PremaratneJ Andrew Hudson
Aug 19, 2008·Applied and Environmental Microbiology·S Thisted LambertzM Lindblad
Apr 14, 2012·International Journal of Food Microbiology·Shahram Hanifian, Sajjad Khani
Mar 7, 2014·International Journal of Food Microbiology·Silvia BonardiFranco Brindani
Feb 9, 2012·International Journal of Food Microbiology·Shahram Hanifian, Sajjad Khani
Sep 21, 2010·Journal of Microbiological Methods·Marc FondrevezMartine Denis
Jun 16, 2014·Food Microbiology·Riikka Laukkanen-NiniosHannu Korkeala
Jul 31, 2012·Veterinary Microbiology·Hristo NajdenskiEls Van Coillie
Jan 24, 2012·Food Microbiology·Cecilia S M Lucero EstradaMaría Esther Escudero
Aug 21, 2016·International Journal of Food Microbiology·Stefanos PetsiosChrissanthy Papadopoulou

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