Detection, Mapping, and Proteotyping of SARS-CoV-2 Coronavirus with High Resolution Mass Spectrometry

ACS Infectious Diseases
Nicholas L DollmanKevin M Downard

Abstract

A high resolution mass spectrometry approach has been applied for the first time to detect and characterize SARS-CoV-2 coronavirus in cell cultured and nasopharyngeal swab specimens. Peptide ions for three of the most abundant structural viral proteins (membrane, nucleocapid, and spike) are detected and assigned directly, by virtue of the high resolution and mass accuracy within the mass maps of whole virus digests, without the need for tandem mass spectrometry (MS/MS). MALDI-MS based approaches offer high sample throughput and speed, compared with those of LC-MS strategies, and detection limits at some 105 copies, or orders of magnitude less with selected ion monitoring, that compete favorably with conventional reverse transcription polymerase chain reaction (RT-PCR) strategies. The detection of signature peptides unique to SARS-CoV-2 coronavirus over those from the influenza virus allows for its unambiguous detection.

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Citations

May 18, 2021·Trends in Analytical Chemistry : TRAC·Justin H Griffin, Kevin M Downard
Sep 4, 2021·Analytical Biochemistry·Hoda IlkhaniSiamak Farhad
Sep 18, 2021·Analytical and Bioanalytical Chemistry·Christian MannKevin M Downard

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Methods Mentioned

BETA
proteotyping
PCR
glycosylation

Software Mentioned

Expasy PeptideMass

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