Detection of 11q13 amplification as the origin of a homogeneously staining region in small cell lung cancer by chromosome microdissection

Genes, Chromosomes & Cancer
J XuN Wang

Abstract

Chromosomal homogeneous staining region (hsr), which is a cytogenetic indication of gene amplification, was found in the pleural effusion (BHII) of a patient with small cell lung cancer (SCLC) after failure of multiple drug treatments. Amplification of band 11q13 was identified as the origin of the hsr by means of chromosomal microdissection combined with G-banding, DNA amplification by polymerase chain reaction, and fluorescence in situ hybridization (micro-FISH). In situ hybridization with the biotin-labeled DNA probe generated from the hsrs of BHII to the cell line BHI, which was established from the lymph node metastasis prior to chemotherapy, revealed the preexistence of 11q13 amplification. This ruled out the possibility of therapeutic induction of the 11q13 amplification. However, the hsr-bearing marker chromosomes were identified by the micro-FISH approach as der(21)(Xqter-->Xq24::hsr(11)(q13)::21p11-->21qter+ ++) in BHII but as der(11)t(3;11)(q21;q13)hsr(11)(q13) in BHI. This suggests that 11q13 DNA sequence amplification may occur first and is then followed by various types of structural rearrangements. FISH analysis with INT2/FGF3 and HST1/FGF4 probes revealed that these protooncogenes were coamplified in the hsrs of...Continue Reading

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Citations

Sep 17, 1998·Genes, Chromosomes & Cancer·J BernardinoB Dutrillaux
Mar 6, 2012·Biochemical and Biophysical Research Communications·Qingchang LiEnhua Wang
Nov 27, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·L Mao
Dec 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Vanita K GuptaTony E Godfrey
Jul 1, 2008·European Journal of Pharmacology·Medhi WangpaichitrNiramol Savaraj
May 15, 2012·Biochimica Et Biophysica Acta·Dorian R A SwartsErnst-Jan M Speel
Oct 31, 2002·American Journal of Medical Genetics·Nancy Wang
May 20, 2017·Oncotarget·Medhi WangpaichitrNiramol Savaraj
Mar 16, 2017·Virchows Archiv : an International Journal of Pathology·Xiuwei ZhangEnhua Wang
Oct 20, 2019·Molecular Cancer Research : MCR·Dan J M NguyenMedhi Wangpaichitr
Jul 22, 2005·Molecular Cancer·Chunjing WuNiramol Savaraj

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