Detection of 2-O-sulfated iduronate and N-acetylglucosamine units in heparan sulfate by an antibody selected against acharan sulfate (IdoA2S-GlcNAc)n

The Journal of Biological Chemistry
Gerdy B ten DamToin H van Kuppevelt

Abstract

The snail glycosaminoglycan acharan sulfate (AS) is structurally related to heparan sulfates (HS) and has a repeating disaccharide structure of alpha-d-N-acetylglucosaminyl-2-O-sulfo-alpha-l-iduronic acid (GlcNAc-IdoA2S) residues. Using the phage display technology, a unique antibody (MW3G3) was selected against AS with a V(H)3, DP 47, and a CDR3 amino acid sequence of QKKRPRF. Antibody MW3G3 did not react with desulfated, N-deacetylated or N-sulfated AS, indicating that reactivity depends on N-acetyl and 2-O-sulfate groups. Antibody MW3G3 also had a high preference for (modified) heparin oligosaccharides containing N-acetylated glucosamine and 2-O-sulfated iduronic acid residues. In tissues, antibody MW3G3 identified a HS oligosaccharide epitope containing N-acetylated glucosamine and 2-O-sulfated iduronic acid residues as enzymatic N-deacetylation of HS in situ prevented staining, and 2-O-sulfotransferase-deficient Chinese hamster ovary cells were not reactive. An immunohistochemical survey using various rat organs revealed a distinct distribution of the MW3G3 epitope, which was primarily present in the basal laminae of most (but not all) blood vessels and of some epithelia, including human skin. No staining was observed in t...Continue Reading

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Citations

Aug 27, 2004·Glycobiology·Eun Ji JooYeong Shik Kim
Sep 21, 2011·Glycobiology·Michiko N Fukuda
Jan 12, 2007·Pediatric Surgery International·S M ThompsonP D Losty
May 4, 2010·Experimental Cell Research·Xander M R van WijkToin H van Kuppevelt
May 17, 2005·Journal of Thrombosis and Haemostasis : JTH·W C Aird
Jun 22, 2010·Journal of Cellular Biochemistry·Eun Ji JooYeong Shik Kim
Dec 24, 2005·The Journal of Biological Chemistry·Gerdy B Ten DamToin H van Kuppevelt
Aug 9, 2018·Scientific Reports·David F ThiekerRobert J Woods
Nov 12, 2021·The Journal of Physical Chemistry. B·Vivek Govind KumarMahmoud Moradi

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