PMID: 3754771May 21, 1986Paper

Detection of 4-hydroxynonenal and other lipid peroxidation products in the liver of bromobenzene-poisoned mice

Biochimica Et Biophysica Acta
A BenedettiM Comporti

Abstract

Lipid peroxidation in cellular membranes leads to the formation of toxic aldehydes. One product provided with particular reactivity has been identified as 4-hydroxynonenal and thoroughly studied as one of the possible mediators of the cellular injury induced by pro-oxidants. In the present study we have searched for the presence of 4-hydroxynonenal and other lipid peroxidation products in the liver of bromobenzene-poisoned mice, since under this experimental condition the level of lipid peroxidation is much greater than in the case of CCl4 or BrCCl3 hepatotoxicity. 4-Hydroxynonenal was looked for in liver extracts as either free aldehyde or its 2,4-dinitrophenylhydrazone derivative. In both cases, by means of thin-layer chromatography (TLC) and high-pressure liquid chromatography, a well resolved peak corresponding to the respective standards (free aldehyde or 2,4-dinitrophenylhydrazone derivative) was obtained. Total carbonyls present in the liver of intoxicated animals were detected as 2,4-dinitrophenylhydrazone derivatives. The hydrazones were pre-separated by TLC into three fractions according to different polarity (polar, non-polar, fraction I, and non-polar, fraction II). The amounts of carbonyls present in each fraction ...Continue Reading

References

May 15, 1979·The Biochemical Journal·A BenedettiM Comporti
Jul 1, 1979·Biochemical Pharmacology·A WendelK H Konz
Jan 1, 1978·Methods in Enzymology·J A Buege, S D Aust
May 13, 1982·Biochimica Et Biophysica Acta·A BenedettiM Comporti

❮ Previous
Next ❯

Citations

Jan 1, 1989·Chemico-biological Interactions·M Comporti
Jan 1, 1989·Free Radical Biology & Medicine·H Esterbauer, H Zollner
Jan 1, 1989·Free Radical Biology & Medicine·G Witz
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Mar 12, 2003·Biochemical Pharmacology·Wilbert H M HeijneBen van Ommen
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Uchida, E R Stadtman
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K UchidaE R Stadtman
Jun 7, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sohei ItoKoji Uchida
Jul 1, 1995·The Journal of Clinical Investigation·H TsukamotoG M Brittenham
Apr 15, 1991·Biochemical Pharmacology·A PompellaM Comporti
Feb 19, 2002·Kidney International·Marilena MisterGiuseppe Remuzzi
May 31, 1990·Biochemical and Biophysical Research Communications·J K BeckmanH L Greene
Aug 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S KamimuraH Tsukamoto
Jan 11, 2019·Journal of Cellular Biochemistry·Gulsah AlbayrakIrem Dogan Turacli
Aug 30, 2008·American Journal of Respiratory and Critical Care Medicine·Ron DoSusan M Kennedy
Nov 1, 1987·Chemistry and Physics of Lipids·G PoliM U Dianzani
Nov 1, 1987·Chemistry and Physics of Lipids·M Comporti
Jan 1, 1993·Molecular Aspects of Medicine·M Comporti
Nov 13, 2007·Free Radical Biology & Medicine·Mario ComportiConcetta Gardi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.