Detection of a knockdown resistance mutation associated with permethrin resistance in the body louse Pediculus humanus corporis by use of melting curve analysis genotyping.

Journal of Clinical Microbiology
Rezak DraliPhilippe Brouqui

Abstract

Louse-borne diseases are prevalent in the homeless, and body louse eradication has thus far been unsuccessful in this population. We aim to develop a rapid and robust genotyping method usable in large field-based clinical studies to monitor permethrin resistance in the human body louse Pediculus humanus corporis. We assessed a melting curve analysis genotyping method based on real-time PCR using hybridization probes to detect the M815I-T917I-L920F knockdown resistance (kdr) mutation in the paraorthologous voltage-sensitive sodium channel (VSSC) α subunit gene, which is associated with permethrin resistance. The 908-bp DNA fragment of the VSSC gene, encoding the α subunit of the sodium channel and encompassing the three mutation sites, was PCR sequenced from 65 lice collected from a homeless population. We noted a high prevalence of the 3 indicated mutations in the body lice collected from homeless people (100% for the M815I and L920F mutations and 56.73% for the T917I mutation). These results were confirmed by melting curve analysis genotyping, which had a calculated sensitivity of 100% for the M815I and T917I mutations and of 98% for the L920F mutation. The specificity was 100% for M815I and L920F and 96% for T917I. Melting cu...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Dec 10, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D Raoult, V Roux
Mar 11, 2000·Lancet·O Chosidow
Apr 13, 2000·Annual Review of Entomology·J Hemingway, H Ranson
May 17, 2001·Antimicrobial Agents and Chemotherapy·C C Randegger, H Hächler
Mar 2, 2002·The Lancet Infectious Diseases·D RaoultP Brouqui
Sep 1, 1954·The American Journal of Tropical Medicine and Hygiene·H S HURLBUTA A SALAH
Jan 12, 2005·Medicine·Philippe BrouquiDidier Raoult
Jan 3, 2006·The Journal of Infectious Diseases·Cedric FoucaultPhilippe Brouqui
Jan 9, 2007·Invertebrate Neuroscience : in·Ke Dong
May 10, 2007·IUBMB Life·T G E DaviesM S Williamson
May 6, 2008·The Journal of Antimicrobial Chemotherapy·Sékéné BadiagaPhilippe Brouqui
Feb 7, 2009·The Journal of Molecular Diagnostics : JMD·Elaine Lyon, Carl T Wittwer

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Citations

Sep 11, 2013·Pesticide Biochemistry and Physiology·Frank D RinkevichKe Dong
Aug 17, 2016·BioMed Research International·Abdoul Karim SangaréDidier Raoult
Mar 29, 2014·Experimental Biology and Medicine·Samson S Y WongKwok-Yung Yuen
Aug 7, 2018·Infectious Diseases of Poverty·Waleed M Sweileh
Sep 2, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Maureen LarochePhilippe Parola
Jun 10, 2021·Journal of Medical Entomology·Fereshteh Ghahvechi KhalighSaber Gholizadeh

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