Detection of a New and Unusual Isolate of Plum pox virus in Plum (Prunus domestica)

Plant Disease
D JamesS Hayes

Abstract

Plum pox virus (PPV) isolate 3174-01 was detected by triple-antibody sandwich enzyme-linked immunosorbent assay using the universal PPV monoclonal antibody (MAb) 5B as the secondary antibody, and by reverse-transcription polymerase chain reaction (RT-PCR) using primers that amplify a 243-bp fragment in the C-terminus of the coat protein coding region. The restriction sites RsaI and AluI were absent from this fragment, which is a feature unique to PPV-C isolates. The restriction sites in 3174-01 were replaced by GTAA/GTGA and GGCA, respectively. There was 95 to 99, 94, 91, and 92 to 94% identity of the 243-bp fragment of 3174-01 with the corresponding region of the strains C, D, EA, and M, respectively. Attempts to detect the virus by RT-PCR using strain C-specific primers in three different approaches were unsuccessful. All molecular techniques assessed in attempting to strain type isolate 3174-01 gave negative results, or results inconsistent for D or M in the case of P3-6K1 restriction fragment length polymorphism analysis. Isolate 3174-01 reacted in Western blot assay with MAb 5B, with an estimated molecular mass of 32 kDa. No reaction was observed with D-, M-, EA-, or C-specific monoclonal antibodies in Western blot or enzy...Continue Reading

References

Aug 1, 1991·Journal of Virological Methods·T WetzelJ Dunez
Dec 11, 1989·Nucleic Acids Research·P Y TeycheneyJ Dunez
Mar 1, 1989·The Journal of General Virology·E MaissH W Katinger
Jun 1, 1989·Virus Research·S LaínJ A García
Jan 1, 2001·Plant Disease·D ThompsonD James
Jul 1, 1997·Plant Disease·A CrescenziA Hadidi

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Citations

Aug 17, 2019·The Journal of General Virology·Mohammad HajizadehMiroslav Glasa
Dec 4, 2012·Viruses·Jiri SochorRene Kizek
Oct 11, 2015·Journal of Economic Entomology·D Thomas LoweryRobert G Foottit
Aug 1, 2012·Plant Disease·Anna ShevelevaSergei Chirkov

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