Detection of a New Luteovirus in Imported Nectarine Trees: A Case Study to Propose Adoption of Metagenomics in Post-Entry Quarantine

Phytopathology
Sudeep BagMysore R Sudarshana

Abstract

In spring 2013, 5-year-old nectarine (Prunus persica) trees, grafted on peach rootstock Nemaguard, were found stunted in a propagation block in California. These trees had been propagated from budwood of three nectarine cultivars imported from France and cleared through the post-entry quarantine procedure. Examination of the canopy failed to reveal any obvious symptoms. However, examination of the trunks, after stripping the bark, revealed extensive pitting on the woody cylinder. To investigate the etiological agent, double-stranded RNA was extracted from bark scrapings from the scion and rootstock portions, and a cDNA library was prepared and sequenced using the Illumina platform. BLAST analysis of the contigs generated by the de novo assembly of sequence reads indicated the presence of a novel luteovirus. Complete sequence of the viral genome was determined by sequencing of three overlapping cDNA clones generated by reverse transcription-polymerase chain reaction (RT-PCR) and by rapid amplification of the 5'- and 3'-termini. The virus genome was comprised of 4,991 nucleotides with a gene organization similar to members of the genus Luteovirus (family Luteoviridae). The presence of the virus, tentatively named Nectarine stem p...Continue Reading

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Citations

Aug 6, 2016·Annual Review of Phytopathology·Robert R MartinIoannis E Tzanetakis
Sep 13, 2016·Frontiers in Microbiology·Ahmed HadidiMarina Barba
Nov 7, 2016·Archives of Virology·D E V VillamorK C Eastwell
Feb 1, 2018·Scientific Reports·Yeonhwa JoWon Kyong Cho
Feb 26, 2019·Phytopathology·D E V VillamorI E Tzanetakis
Jul 3, 2020·Plants·Julia MinickaBeata Hasiów-Jaroszewska
Feb 28, 2020·Viruses·Wycliff M KinotiFiona E Constable
Jul 4, 2020·Archives of Virology·Maryam KhaliliArmelle Marais
Sep 19, 2019·Letters in Applied Microbiology·Y XuS Li
Jun 5, 2017·Virus Genes·João Marcos Fagundes SilvaThor Vinícius Martins Fajardo
Jul 7, 2020·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Luisa Rubino
Feb 16, 2021·Frontiers in Microbiology·Muhammad Shafiq ShahidAbdullah M Al-Sadi

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