Detection of Aβ oligomers based on magnetic-field-assisted separation of aptamer-functionalized Fe3 O4 magnetic nanoparticles and BaYF5 :Yb,Er nanoparticles as upconversion fluorescence labels

Talanta
Ling-Feng JiangLin Wu

Abstract

A sensitive and stable bioassay for the detection of Aβ oligomer (Aβo), a potentially promising candidate biomarker for Alzheimer's disease (AD) diagnosis, was developed using Fe3O4 magnetic nanoparticles (MNPs) as the recognition and concentration elements and BaYF5:Yb,Er upconversion nanoparticles (UCNPs) as highly sensitive labels, conjugated with the Aβo aptamer (DNA1) and the complementary oligonucleotide of the Aβo aptamer (DNA2), respectively. The DNA1 hybridized with DNA2 to form the duplex structure on the surface of the MNPs/UCNPs nanocomposites probe. When the target Aβo was introduced, the aptamer DNA1 preferentially bound with Aβo and caused the dissociation of some complementary DNA2, liberating some UCNP-labeled complementary DNA2 and leading to a decreased upconversion fluorescent intensity on the surface of MNPs. The decreased fluorescence intensity of UCNPs was related to the concentration of Aβo in the range of 0.2-15nM with a detection limit of 36 pM. The developed method then was successfully applied to measure Aβo in artificial cerebrospinal fluid. Benefiting from the magnetic separation and concentration effect of MNPs, the high sensitivity of UCNPs, as well as the selectivity and stability of the aptamer...Continue Reading

Citations

Nov 17, 2017·International Journal of Molecular Sciences·Pascal RöthlisbergerMarcel Hollenstein
Aug 2, 2018·Small·Jiajia ZhouJean-Claude G Bünzli
Nov 14, 2019·Chemical Society Reviews·Mostafa Kamal MasudYusuke Yamauchi
Aug 26, 2020·Mikrochimica Acta·Hong-Xia RenYing Zhang
Nov 26, 2020·Nanotechnology·Divya GopalanSrinivas Mutalik
Jan 19, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zeyu SongXiaoying Tang
Apr 6, 2021·Advances in Colloid and Interface Science·Ashis TripathyFilipe Samuel Silva
Apr 11, 2021·Journal of Colloid and Interface Science·Diego Mendez-GonzalezSonia Melle

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