PMID: 6165021Mar 1, 1981Paper

Detection of a recombinant murine leukemia virus-related glycoprotein on virus-negative thymoma cells

Proceedings of the National Academy of Sciences of the United States of America
P J FischingerJ H Elder

Abstract

X-irradiation of outbred Swiss mice resulted in the development of virus-free thymomas. When put in culture, a lymphoblastic cell line (NIXT) expressed neither particles nor infectious virus but supported the growth of pure ecotropic murine leukemia viruses (MuLVs) without generating any envelope recombinant (RM) MuLV in more than 20 months of culture. These cells did not support the growth of RM-MuLVs and completely excluded the entry of all RM-MuLV pseudotypes of murine sarcoma virus, suggesting specific viral interference. Radioimmunocompetition and immunofluorescence assays with broadly reactive anti-MuLV-p30 and -gp70 antisera were negative. However, in immunofluorescence with antisera specifically reactive against RM-MuLV gp70, about 5-20% of the population of parental cells or their clones were positive. NIXT cells treated with this antiserum bound protein A and exhibited complement-dependent cytotoxicity as assessed by several assays. NIXT cells could partially absorb neutralizing antibody specific for RM-MuLVs. Based on radioimmunoprecipitation tests, NIXT cells bore, on the cell surface, a glycosylated protein (gp70) reactive with RM subgroup as well as some group-specific anti-gp70 antisera. The glycoprotein was also...Continue Reading

References

Jan 1, 1977·Medical Microbiology and Immunology·P J FischingerD P Bolognesi
Jan 1, 1975·Cold Spring Harbor Symposia on Quantitative Biology·S K ChattopadhyayW P Rowe
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A DeclèveH S Kaplan
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J W HartleyW P Rowe
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J RommelaereN Hopkins
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P J FischingerD P Bolognesi
Feb 1, 1972·The Journal of Experimental Medicine·W P Rowe, T Pincus
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S A Aaronson, J R Stephenson
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M StrandJ T August
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Feb 19, 1971·Nature·R H BassinP J Fischinger
Jan 1, 1951·Proceedings of the Society for Experimental Biology and Medicine·L GROSS

❮ Previous
Next ❯

Citations

Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F A van der HoornH P Bloemers
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A De RossiL Chieco-Bianchi
Apr 1, 1990·International Journal of Radiation Biology·M JanowskiP G Strauss
Jan 1, 1986·Leukemia Research·M JanowskiP Reddy
Jan 1, 1989·Molecular Carcinogenesis·R S NairnJ P Allison

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.