Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer

Biomaterials
Masayo OgisoToshimi Shimizu

Abstract

To develop a simple and inexpensive DNA detection method, we prepared a molecularly imprinted polymer (MIP) gel for recognizing a specific double-stranded DNA (dsDNA) target sequence in MIP gel electrophoresis (MIPGE). During MIPGE, migration of the target sequence of dsDNA should be hindered by the capture effect of the binding sites in the MIP gel. This migration hindrance of target dsDNA was determined by plotting the relationship between the migration distance in the MIP gel and that in polyacrylamide gel, commonly used in gel electrophoresis. Using this plot, detection of a target dsDNA from a mixture of different-sized dsDNA fragments was achieved. Moreover, we found the detection method successfully distinguished between a target and its base-pair substitutes. These results suggest that MIPGE could be employed for detection of a target dsDNA sequence.

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Citations

Nov 15, 2008·Chemical Communications : Chem Comm·Ruizhuo OuyangHuangxian Ju
Jun 20, 2012·Biosensors & Bioelectronics·Kei-ichi OgawaNorihiko Minoura
Dec 15, 2015·Biotechnology Advances·Zofia IskierkoWlodzimierz Kutner
Oct 1, 2008·International Journal of Pharmaceutics·Mark E Byrne, Vishal Salian
Oct 27, 2015·Journal of Molecular Modeling·Zhengqiang DaiRuifa Jin
Jan 16, 2008·Journal of Separation Science·Wei YaoZhixian Gao
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Mar 30, 2017·Sensors·Erkut YılmazLokman Uzun
Feb 15, 2019·Small·Zijie Zhang, Juewen Liu
May 22, 2019·Macromolecular Rapid Communications·Nan ZhangXiaoling Hu
Oct 27, 2021·Journal of Molecular Recognition : JMR·Hazim F El SharifSubrayal M Reddy

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