PMID: 22338581Feb 18, 2012Paper

Detection of abacavir hypersensitivity by ELISpot method

Inflammation & Allergy Drug Targets
Stefan EsserMonika Lindemann

Abstract

The antiretroviral agent abacavir can cause hypersensitivity reaction (HSR) and the presence of HLA-B*57:01 is predictive of abacavir-HSR in Caucasian HIV-infected patients. However, abacavir-HSR also occurs in HLA-B*57:01 negative patients. In these patients, a safe diagnostic tool to dissect clinically suspected HSR against abacavir from adverse reactions against co-administered drugs is mandatory, and abacavir-ELISpot was evaluated. Peripheral blood mononuclear cells from 87 HIV patients were stimulated by abacavir and the production of interferon-γ to the ELISpot was determined. Abacavir treated patients with HSR [confirmed (n=5) or suspected (n=12)] vs without HSR (n=42) displayed significantly higher numbers of abacavir-specific cells (82.3±23.0 or 10.5±4.5 vs -0.5±1.0 spot forming cells per million PBMC, p < .005 each). In conclusion, we established the first abacavir-specific ELISpot. According to our preliminary data, a negative abacavir-ELISpot nearly excludes HSR against abacavir. Thereby the ELISpot may facilitate the decision to continue or withdraw abacavir treatment.

Citations

Jun 11, 2016·The Journal of Allergy and Clinical Immunology. in Practice·Jason A TrubianoAnton Y Peleg
Mar 19, 2016·Allergy·C MayorgaUNKNOWN In vitro tests for Drug Allergy Task Force of EAACI Drug Interest Group
Oct 27, 2016·Current Opinion in Infectious Diseases·Katherine C KonvinseJason A Trubiano
Mar 4, 2017·The British Journal of Dermatology·N R AdlerE J Phillips
Mar 21, 2020·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Xiang ZhangXiao-Ming Qin
May 4, 2021·Journal of Immunological Methods·Grzegorz PorebskiRadoslaw Spiewak

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