PMID: 8444644Feb 1, 1993Paper

Detection of Aeromonas hydrophila in food with an enzyme-linked immunosorbent assay

The Journal of Applied Bacteriology
S MerinoJ M Tomás

Abstract

A microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for the detection of Aeromonas hydrophila serotype O: 11 (highly virulent strains). The assay utilizes a detector antibody which shows no cross-reactions with Aeromonas strains other than serotype O: 11 or non-Aeromonas competing organisms. The detector antibody is mixed with the sample and incubated for 1 h, microcentrifuged and the supernatant fluid (unadsorbed antibody) titred in a microtitre plate coated with A. hydrophila cells from serotype O: 11. All the A. hydrophila strains from serotype O: 11 tested reacted strongly with the detector antibody. Also by culturing and performing the immunoassay with the detector antibody we established and quantified the presence of A. hydrophila O: 11 in different foods.

Citations

Dec 1, 1993·International Journal of Food Microbiology·S M Kirov
Dec 1, 1995·International Journal of Food Microbiology·S MerinoJ M Tomas
Nov 10, 2013·Journal of Agricultural and Food Chemistry·Sun Hee CheongPyo-Jam Park
Jul 2, 1998·Applied and Environmental Microbiology·K KooH E Swaisgood

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