Detection of African swine fever, classical swine fever, and foot-and-mouth disease viruses in swine oral fluids by multiplex reverse transcription real-time polymerase chain reaction

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Frederic R GrauMangkey A Bounpheng

Abstract

African swine fever (ASF), classical swine fever (CSF), and foot-and-mouth disease (FMD) are highly contagious animal diseases of significant economic importance. Pigs infected with ASF and CSF viruses (ASFV and CSFV) develop clinical signs that may be indistinguishable from other diseases. Likewise, various causes of vesicular disease can mimic clinical signs caused by the FMD virus (FMDV). Early detection is critical to limiting the impact and spread of these disease outbreaks, and the ability to perform herd-level surveillance for all 3 diseases rapidly and cost effectively using a single diagnostic sample and test is highly desirable. This study assessed the feasibility of simultaneous ASFV, CSFV, and FMDV detection by multiplex reverse transcription real-time polymerase chain reaction (mRT-qPCR) in swine oral fluids collected through the use of chewing ropes. Animal groups were experimentally infected independently with each virus, observed for clinical signs, and oral fluids collected and tested throughout the course of infection. All animal groups chewed on the ropes readily before and after onset of clinical signs and before onset of lameness or serious clinical signs. ASFV was detected as early as 3 days postinoculatio...Continue Reading

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Citations

Nov 28, 2015·Preventive Veterinary Medicine·Nitipong HomwongDouglas Marthaler
Jun 1, 2016·Frontiers in Veterinary Science·Carolina StenfeldtJonathan Arzt
Sep 17, 2016·Transboundary and Emerging Diseases·C SenthilkumaranC Nfon
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Jul 18, 2018·Transboundary and Emerging Diseases·Yaowalak PanyasingRoongroje Thanawongnuwech
Jul 21, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hanna Turlewicz-PodbielskaMałgorzata Pomorska-Mól
Sep 24, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·John FlanneryCarrie Batten
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Jun 26, 2021·Transboundary and Emerging Diseases·Richard BradhurstKeith Sumption
Jul 29, 2021·Analytical Chemistry·Liben ChenTza-Huei Wang

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Methods Mentioned

BETA
nucleic acid purification
electrophoresis
PCR
nucleic

Software Mentioned

RSQ Excel

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