Detection of all known parechoviruses by real-time PCR

Journal of Clinical Microbiology
W Allan NixM Steven Oberste

Abstract

The Parechovirus genus of the Picornaviridae family contains two species, Human parechovirus (HPeV) and Ljungan virus (LV). The HPeVs (including the former echoviruses 22 and 23, now HPeV type 1 (HPeV1) and HPeV2, respectively) cause a wide spectrum of disease, including aseptic meningitis, gastroenteritis, encephalitis, acute respiratory illness, and neonatal sepsis-like disease. The LVs were isolated from bank voles in Sweden during a search for an infectious agent linked to fatal myocarditis cases in humans. Because of the decline in use of cell culture and neutralization to investigate enterovirus-like disease, very few laboratories currently have the capability to test for parechoviruses. We have developed a real-time reverse transcription-PCR (RT-PCR) assay for detection of all known members of the genus Parechovirus. The assay targets the conserved regions in the 5' nontranslated region (5'NTR) of the parechovirus genome and can detect both HPeVs and LVs, unlike other published parechovirus 5' NTR assays, which only detect known HPeVs or only LVs. HPeV and LV can be differentiated by sequencing the 5'NTR real-time RT-PCR amplicon, when needed. The assay is approximately 100 times more sensitive than cell culture and may ...Continue Reading

References

Jul 1, 1995·The Pediatric Infectious Disease Journal·C ShimizuJ C Burns
Mar 1, 1997·Journal of Clinical Microbiology·S J ReadC R Bangham
Jul 24, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Joki-Korpela, T Hyypiä
May 21, 1999·Journal of Medical Virology·M S ObersteM A Pallansch
Feb 2, 2000·Reviews in Medical Virology·G StanwayT Hyypiä
Oct 9, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Vincent LegaySameer Khalfan
Mar 26, 2003·The Journal of General Virology·E Susanne JohanssonA Michael Lindberg
May 15, 2003·International Journal of Experimental Diabesity Research·Bo NiklassonAke Lernmark
Dec 1, 1957·American Journal of Public Health and the Nation's Health
Jan 1, 1961·Archiv für die gesamte Virusforschung·R WIGAND, A B SABIN
Feb 11, 2004·The Journal of General Virology·Miyabi ItoKenji Sakae
Mar 8, 2005·Journal of Clinical Microbiology·Pia JokelaTimo Hyypiä
Dec 16, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·K S M BenschopK C Wolthers
Dec 21, 2006·Emerging Infectious Diseases·Kimberley S M BenschopKatja C Wolthers
Mar 6, 2007·Birth Defects Research. Part A, Clinical and Molecular Teratology·Bo NiklassonWilliam Klitz
Jun 8, 2007·Emerging Infectious Diseases·Kanako WatanabeMasahiro Fujii
Mar 21, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Kimberley BenschopMarcel Beld
Mar 21, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Gerda T NoordhoekAfke H Brandenburg

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Citations

Jun 13, 2013·The Journal of Infectious Diseases·Preeti ChhabraJan Vinjé
Aug 13, 2013·The Journal of Infectious Diseases·Albert E BarskeyGregory S Wallace
Jun 2, 2009·The Pediatric Infectious Disease Journal·Rebecca E LevorsonJoseph M Campos
Aug 28, 2009·The Pediatric Infectious Disease Journal·Rebecca E Levorson, Barbara A Jantausch
Nov 30, 2012·The Pediatric Infectious Disease Journal·Kensuke ShojiAkihiko Saitoh
Aug 30, 2008·Journal of Clinical Microbiology·H HarvalaP Simmonds
Sep 28, 2012·Journal of Clinical Microbiology·Justin SharpRangaraj Selvarangan
Apr 8, 2011·Journal of Clinical Microbiology·Maja KodaniBarry S Fields
Oct 26, 2012·Journal of Virology·M Steven ObersteLisa Jones-Engel
Apr 9, 2013·Archives of Disease in Childhood. Fetal and Neonatal Edition·Rimma MelamedPhillip I Tarr
Nov 7, 2012·Pediatrics·Joshua M ColvinGregory A Storch
Oct 26, 2012·Emerging Infectious Diseases·Katsumi MizutaTadayuki Ahiko
Sep 21, 2013·Emerging Infectious Diseases·Giulia CampaniniFausto Baldanti
Aug 19, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Oskar SkogOlle Korsgren
Jun 6, 2014·European Journal of Pediatrics·Simon B DrysdaleAnne Greenough
Nov 28, 2013·Journal of Neurovirology·Pooneh RahimiMehdi Shafiee Ardestani
Aug 5, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Beth WaltersXiaotian Zheng
Jul 9, 2011·Advances in Pediatrics·José R Romero, Rangaraj Selvarangan
Sep 2, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Yuan-Pin HuangJyh-Yuan Yang
May 18, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·W Allan NixM Steven Oberste
Apr 18, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·H Harvala, P Simmonds
Jun 27, 2015·Journal of Neuropathology and Experimental Neurology·Stephanie J BisselClayton A Wiley
May 14, 2016·Pediatrics International : Official Journal of the Japan Pediatric Society·Jun NireiAkihiko Saitoh
Oct 11, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Zornitsa MladenovaHiroshi Ushijima
Feb 18, 2012·Journal of Medical Virology·Antonio PirallaFausto Baldanti
May 16, 2012·Journal of Medical Virology·Tuula PelkonenAnne Pitkäranta
Jun 25, 2013·Journal of Medical Virology·Marja-Leena Simonen-TikkaMerja Roivainen
Sep 4, 2012·Pediatric Dermatology·Kensuke ShojiAkihiko Saitoh
Oct 21, 2015·Emerging Infectious Diseases·Yuta AizawaAkihiko Saitoh
Sep 15, 2015·Nature Medicine·Efrem S LimLori R Holtz
Sep 12, 2015·Journal of Medical Microbiology·Seiji P YamamotoNobuhiro Iritani
Jun 28, 2013·The Journal of General Virology·W Allan NixM Steven Oberste

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