PMID: 7541405Apr 1, 1995Paper

Detection of all serotypes of human astrovirus by the polymerase chain reaction

Journal of Virological Methods
T O JonassenB Grinde

Abstract

A reverse transcription (RT) and polymerase chain reaction (PCR) was designed for the detection of astroviruses based on a conserved nucleotide sequence in the 3'-end of the genome of the 7 known serotypes of human astrovirus. Thirty-eight samples found to contain astrovirus by electron microscopy (EM) were used for evaluation of the assay. The samples were dialyzed for 1 h to remove potential low molecular weight inhibitors of the RT-PCR. Immediately before RT, 1 microliters of the samples were incubated at 94 degrees C for 2 min to disrupt the viral particles. Thirty-six of the samples were positive by PCR, including samples of all 7 serotypes. The two samples that were negative, could hve been false positive by EM, or the viral RNA could have been degraded. All other viruses examined, including calici-, rota- and enteroviruses, were negative.

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Citations

Nov 1, 1996·Journal of Medical Virology·K Stene-Johansen, B Grinde
Jul 14, 1998·Journal of Medical Virology·G G GonzálezJ E Ludert
Feb 13, 2001·Journal of Virological Methods·G M BelliotS S Monroe
Aug 18, 2000·International Journal of Food Microbiology·D Lees
Aug 15, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·B FoleyJ G Morgan
Apr 10, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Aude Marie-CardineClaudine Buffet-Janvresse
Mar 27, 1999·The Pediatric Infectious Disease Journal·D K MitchellM J Carter
Jan 22, 2000·Journal of Virology·D M Bass, S Qiu
May 1, 2008·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Hsiao-Chuan LinLi-Min Huang
Apr 12, 2001·The Journal of General Virology·C M JonassenB Grinde
Mar 21, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rittwika BhattacharyaTriveni Krishnan
May 11, 2017·Viruses·Celeste Donato, Dhanasekaran Vijaykrishna
Jul 28, 2016·Diseases·Torstein Tengs, Christine M Jonassen
Aug 1, 1997·Applied and Environmental Microbiology·F X AbadA Bosch

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