Detection of amino acid and peptide transmitters in physiologically identified brainstem cardiorespiratory neurons

Autonomic Neuroscience : Basic & Clinical
Patrice G GuyenetJohnny R Simmons

Abstract

Most of the CNS neurons that regulate circulation and respiration reside in regions of the brain characterized by extreme cellular heterogeneity (nucleus of the solitary tract, reticular formation, parabrachial nuclei, periaqueductal gray matter, hypothalamus, etc.). The chemical neuroanatomy of these regions is correspondingly complex and teasing out specific circuits in their midst remains a problem that is usually very difficult if not impossible to solve by conventional tract-tracing methods, Fos methodology or electrophysiology in slices. In addition, identifying the type of amino acid or peptide transmitter used by electrophysiologically recorded neurons has been until recently an especially difficult task either for lack of a specific marker or because such markers (many peptides for example) are exported to synaptic terminals and thus undetectable in neuronal cell bodies. In this review, we describe a general purpose method that solves many of these problems. The approach combines juxtacellular labeling in vivo with the histological identification of mRNAs that provide definitive neurochemical phenotypic identification (e.g. vesicular glutamate transporter 1 or 2, glutamic acid decarboxylase). The results obtained with ...Continue Reading

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Citations

Jan 16, 2010·Journal of Applied Physiology·Patrice G GuyenetRoy Kanbar
Mar 17, 2007·The International Journal of Neuroscience·Y LiangDavid T Yew
Jan 22, 2009·Respiratory Physiology & Neurobiology·Juan Luis Ribas-SalgueiroRosario Pásaro
May 27, 2010·Journal of Hypertension·Alain G Dupont, Sofie Brouwers
Jun 9, 2006·Nature Reviews. Neuroscience·Patrice G Guyenet
Nov 13, 2015·Brain Structure & Function·Ruth L StornettaPatrice G Guyenet
Feb 15, 2007·The Journal of Comparative Neurology·Pedro Barroso-ChineaJosé L Lanciego

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