Detection of an Abundant Plant-Based Small RNA in Healthy Consumers

PloS One
Jian YangKendal D Hirschi

Abstract

The mechanisms of delivery of plant small RNAs to consumers must be investigated in order to harness this technology to positively impact biotechnology. Two groups have used honeysuckle (Lonicera japonica) feeding regimes to detect a plant-based small RNA, termed MIR2911, in sera. Meanwhile, numerous groups have failed to detect dietary plant-based small RNAs in consumers. Here we catalog levels of MIR2911 in different herbs, and suggest that in particular herb MIR2911 levels are elevated. Feeding these different herb-based diets to mice, we found MIR2911 levels in the sera and urine were associated with dietary intake levels. Abundance was not the sole determinate of apparent RNA bioavailability, as gavage-feeding large-doses of synthetic MIR2911 permitted only small transient increases in serum levels. Dietary MIR2911 were not modified in circulation by association with the host's RNA-induced silencing complex, as the RNA did not co-immunoprecipitate with AGO2. The stability of dietary MIR2911 in circulation differed from synthesized small RNAs, as tail vein administration of various synthetic plant-based small RNAs resulted in rapid clearance. However, synthetic MIR2911 appeared to be more stable than the other plant miRNAs ...Continue Reading

Associated Clinical Trials

Nov 14, 2018·Cristina Rodriguez-Padilla

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Citations

Sep 9, 2016·Scientific Reports·Chiara PastrelloIgor Jurisica
Dec 28, 2016·International Journal of Molecular Sciences·Anna Lukasik, Piotr Zielenkiewicz
Jun 3, 2016·Scientific Reports·Jian YangKendal D Hirschi
Apr 7, 2017·Scientific Reports·Yi LuoMingzhou Li
Apr 5, 2018·Science China. Life Sciences·Wen ZhangZhen Zhou
Sep 1, 2017·PLoS Genetics·Kegan ZhuXi Chen
Nov 27, 2018·RNA Biology· SanchitaPrabodh Kumar Trivedi
May 24, 2019·Advances in Nutrition·Lin ZhangYong-Liang Zhang
Aug 31, 2020·Advances in Nutrition·Abdul Fatah A SamadMuhammad Sajad
Sep 24, 2020·Annual Review of Nutrition·Elizabeth M McNeill, Kendal D Hirschi
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Jun 8, 2017·International Journal of Molecular Sciences·Dinesh KumarJayashankar Das
Jun 6, 2019·American Journal of Physiology. Cell Physiology·Meng LiYong-Liang Zhang
Jan 9, 2020·Cell & Bioscience·Bernardetta LeddaMariangela Miele
Oct 1, 2020·PeerJ·Fermín Mar-AguilarDiana Reséndez-Pérez
Mar 21, 2017·Molecular Nutrition & Food Research·Jian YangKendal D Hirschi
Jul 12, 2017·Genes & Nutrition·Kenneth W Witwer, Chen-Yu Zhang
Mar 7, 2018·Genes & Nutrition·Jian YangKendal D Hirschi
Feb 6, 2021·ExRNA·Jennifer K SpinlerKendal D Hirschi
Oct 22, 2020·Seminars in Cancer Biology·Alberto DávalosDavid de Gonzalo-Calvo
Aug 19, 2021·Critical Reviews in Microbiology·Deepansh ModyVibha Rani

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Methods Mentioned

BETA
urine collection
co-immunoprecipitation
immunoprecipitation
amplicon sequencing

Software Mentioned

Excel
Biorad
Biorad CFX software

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