Detection of analytes in mitochondria without interference from other sites based on an innovative ratiometric fluorophore

Chemical Science
Tianbing RenXiao-Bing Zhang

Abstract

Mitochondria are vital organelles that not only produce cellular energy but also participate in many biological processes. Recently, various fluorescent probes have been developed for mitochondrial imaging. However, due to the lack of suitable dyes or strategies, it is difficult for most reported mitochondrial targeting probes to prove whether the analytes they detected are from mitochondria. In addition, positive charge on mitochondrial probes can seriously affect the mitochondrial environment. To address these issues, we herein put forward a novel strategy for probe design based on a smart NIR dye (HDFL) for mitochondrial targeting detection. Compared to general mitochondrial targeting probes that are modified with a target site and a reaction site, the new strategy is to combine the two sites together for a mitochondrial probe that would provide accurate detection of analytes in mitochondria without interference. As a proof of concept, we synthesized a mitochondrial-targetable probe HDFL-Cys for cysteine. Bioimaging studies have shown that the new type of probe HDFL-Cys can first accumulate in mitochondria and then react with the analyte (cysteine) accompanied by the departure of the targeting group (lipophilic cation moieti...Continue Reading

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Citations

Oct 12, 2018·Chemical Communications : Chem Comm·Hong Huang, Yang Tian
Jun 15, 2019·Chembiochem : a European Journal of Chemical Biology·Shuai XiaHaiying Liu
Jul 31, 2019·Chemical Science·Peng GaoBo Tang
Jan 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hua LiuDayong Shi
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shenggang WangXiangming Guan
Nov 14, 2019·Analytical Chemistry·Qichao YaoXiaojun Peng

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Methods Mentioned

BETA
Fluorescence
fluorescence imaging

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