Detection of Anaplasma ovis infection in goats by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay.

Journal of Clinical Microbiology
L W Ndung'uG H Palmer

Abstract

A competitive inhibition enzyme-linked immunosorbent assay (ELISA) based on a major surface protein 5 (MSP5) B-cell epitope conserved among Anaplasma species was used to detect goats infected with Anaplasma ovis. We examined strains of A. ovis isolated from goats in Kenya and demonstrated that MSP5 and the target B-cell epitope, bound by monoclonal antibody ANAF16C1, were conserved. Sera from 149 goats in four regions of Kenya and from 302 goats in six U.S. states were tested for the presence of epitope-specific antibodies with the MSP5 competitive inhibition ELISA. Evidence that the assay can be used to detect A. ovis-infected goats includes the following: (i) 53 goats raised in confinement with arthropod control were all seronegative; (ii) six goats experimentally infected with A. ovis seroconverted at the same time that they developed detectable rickettsemia; (iii) seroconverted goats remained seropositive, consistent with the persistence of A. ovis in goats and the presence of anti-MSP5 antibody in cattle persistently infected with Anaplasma marginale; and (iv) 119 of 127 known A. ovis-infected goats in Kenya were seropositive. A. ovis infection, as determined serologically and by demonstration of infected erythrocytes, in ...Continue Reading

References

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Citations

Oct 1, 2008·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Rafael A N RamosCleber O Soares
Apr 21, 2010·Tropical Animal Health and Production·Alessandra TorinaJosé de la Fuente
Jul 21, 1998·Annals of the New York Academy of Sciences·M V HerreroG M Buening
Jul 21, 1998·Annals of the New York Academy of Sciences·H SahibiW L Goff
Mar 5, 2014·Transboundary and Emerging Diseases·S RennekerU Seitzer
Jan 23, 2019·BMC Genomics·Zhijie LiuKelly A Brayton
May 10, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kathleen L MasonGlen A Scoles
Jul 6, 2017·Vector Borne and Zoonotic Diseases·Nyamsuren OchirkhuuKazuhiko Ohashi
Jun 24, 2018·Tropical Animal Health and Production·J M Martínez-HernándezS Sánchez-Montes
Aug 28, 2018·PLoS Neglected Tropical Diseases·Thomas C MooreGregory C Gray
Apr 3, 2012·Research in Veterinary Science·S Ait HamouA Rhalem
Apr 26, 2021·Acta Tropica·Amaya WatthanadirekPanat Anuracpreeda
Oct 9, 2009·Veterinary Parasitology·Katherine M KocanS A Ewing

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