Detection of Anti-Pentraxin-3 Autoantibodies in ANCA-Associated Vasculitis

PloS One
Amélie SimonJean-François Augusto

Abstract

Pentraxin 3 (PTX3), in common with myeloperoxidase and proteinase 3, is stored in human neutrophil granules and is expressed on apoptotic neutrophil surface. We therefore investigated the presence of anti-PTX3 autoantibodies (aAbs) in the sera of antineutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) patients. Presence of anti-PTX3 autoantibodies was analysed by a specific enzyme-linked immunosorbent assay in sera from 150 patients with microscopic polyangiitis (MPA), granulomatosis with polyangiitis (GPA), and eosinophilic granulomatosis with polyangiitis (EGPA), and in sera of 227 healthy subjects (HS), 40 systemic sclerosis (SSc) patients, and 25 giant cell arteritis patients (GCA). Using indirect immunofluorescence on fixed human neutrophils, we also analyzed the staining pattern associated with the presence of anti-PTX3 aAbs. Anti-PTX3 aAbs were detected in 56 of 150 (37.3%) of the AAV patients (versus 12 of 227 (5.3%) of HS, p<0.001) and, interestingly, in 7 of 14 MPO and PR3 ANCA negative AAV patients. Moreover, by indirect immunofluorescence on fixed neutrophils, anti-PTX3 aAbs gave rise to a specific cytoplasmic fluorescence pattern distinct from the classical cytoplasmic (c-ANCA), perinuclear (p-ANCA...Continue Reading

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Citations

Aug 2, 2016·Clinical Kidney Journal·Juliana Bordignon DraibeAlan David Salama
May 5, 2017·International Reviews of Immunology·Jean-François AugustoPascale Jeannin
May 12, 2017·JRSM Open·Abhilash KoratalaA Ahsan Ejaz
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Mar 6, 2021·Frontiers in Immunology·Benoit BrillandYves Delneste
Oct 21, 2017·Vascular Pharmacology·Matteo CasulaFederico Carbone

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Methods Mentioned

BETA
ELISA
Enzyme-linked immunosorbent assay
Fluorescence
biopsies

Software Mentioned

Prism
GraphPad

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