Detection of anti-preS1 antibodies for recovery of hepatitis B patients by immunoassay

World Journal of Gastroenterology : WJG
Jun WeiZuchuan Zhang

Abstract

To establish a convenient immunoassay method based on recombinant antigen preS1(21-119aa) to detect anti-preS1 antibodies and evaluate the clinical significance of antibodies in hepatitis B. The expression plasmid pET-28a-preS1 was constructed, and a large quantity of preS1(21-119aa) fragment of the large HBsAg protein was obtained. The preS1 fragment purified by Ni(2+)-IDA affinity chromatography was used as coated antigen to establish the indirect ELISA based on streptavidin-biotin system for detection of the anti-preS1 antibodies in sera from HBV-infected patients. For follow-up study, serial sera were collected during the clinical course of 21 HBV-infected patients and anti-preS1 antibodies, preS1 antigen, HBV-DNA and other serological HBV markers were analyzed. preS1(21-119aa) fragment was highly expressed from the plasmid pET-28a-preS1 in a soluble form in E.Coli (30mg.L(-1)), and easily purified to high purity over 90% by one step of Ni(2+)-IDA-sepharose 6B affinity chromatography. The purity and antigenicity of the purified preS1(21-119aa) protein was determined by 150g.L(-1) SDS-PAGE, Western blot and a direct ELISA. Recombinant preS1(21-119aa) protein was successfully applied in the immunoassay which could sensitively...Continue Reading

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Citations

Dec 12, 2003·World Journal of Gastroenterology : WJG·Huseyin GunduzOmer Fehmi Tabak
Oct 16, 2002·World Journal of Gastroenterology : WJG·Jie YanXiao-Zhi Zhong
Apr 6, 2005·World Journal of Gastroenterology : WJG·Wei-Guo HuZu-Chuan Zhang
Mar 13, 2003·World Journal of Gastroenterology : WJG·Zhan-Sheng JiaXue-Fang Bai
Jan 19, 2020·International Journal of Molecular Sciences·Md Golzar HossainKeiji Ueda
Jul 4, 2015·World Journal of Gastroenterology : WJG·Riki ToitaMasaharu Murata
Apr 27, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yingjie BianHua Peng
Sep 13, 2003·World Journal of Gastroenterology : WJG·Ke-Xia WangChao-Pin Li

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