PMID: 2511357Apr 1, 1989Paper

Detection of anti-SSA/Ro and SSB/La antibodies using the immunoblotting method and clinical analysis

Nihon Hifuka Gakkai zasshi. The Japanese journal of dermatology
Y Hamasaki

Abstract

Sera from 84 patients with connective tissue diseases and 30 healthy volunteers were examined for the presence of anti-SSA/Ro, SSB/La antibodies using immunoblotting techniques, and a comparative study of the results of the double immunodiffusion technique was performed. The SSA/Ro and SSB/La antigens were prepared from human spleen, rabbit thymus acetone powder, and the KB cell line, a human epithelial cell line. By immunoblotting, the SSA/Ro antibody was shown to react mainly with 58 KD peptide, while the SSB/La antibody reacted with 42 KD and 40 KD peptides. Some SSA/Ro positive antisera were also reactive with 64 KD peptide. Using the immunoblotting method, we have found that over 83% of SLE patients possess the SSA/Ro antibody, and 41% possess the SSB/La antibody. The relationship between the presence of these antibodies and SLE abnormalities was examined. No abnormality was seen with significant frequency among patients in the SSA/Ro positive group of SLE. The SLE patients with SSB/La antibody were characterized by a low prevalence of oral ulcers, a low titer for nDNA antibody, and presence of the rheumatoid factor and the anti-Sm antibody. In addition, the SLE patients with an autoantibody reactive with an additional 64 ...Continue Reading

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