Detection of antibodies and antigenemia in leukemic patients with candidiasis by enzyme-linked immunosorbent assay

The Journal of Infectious Diseases
K L MeckstrothL Kaufman

Abstract

Standard tests and two enzyme-linked immunosorbent assays (ELISAs) were compared for detecting systemic candidiasis in 92 leukemic patients. Antibodies to Candida were detected by immunodiffusion (ID), latex agglutination (LA), and indirect ELISA; antigenemia was evaluated by indirect ELISA inhibition. In 48 patients without candidiasis (group 1), sera from 27% reacted in ID and 13% in LA tests; titers of antibody by ELISA occurred in all patients. In 34 patients without disseminated candidiasis (groups 2 and 3), Candida species were isolated; sera from 35% and 32% were positive in the ID and the LA test, respectively. In nine of 10 patients with disseminated candidiasis (group 4), LA and ID tests were negative. Seven patients in groups 1-3 without disseminated candidiasis were antigenemic (false-positive rate, 9%). Antigenemia was detected in seven of 10 patients with disseminated candidiasis; in the remaining three patients sera were drawn too early to be of diagnostic value. Thus, monitoring of immunosuppressed patients for mannan antigenemia is useful for the detection of disseminated candidiasis.

Citations

Jan 1, 1994·Journal of Clinical Laboratory Analysis·K MitsutakeH Koga
Nov 1, 1992·Mycopathologia·M A Pfaller
Apr 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L de Repentigny
Aug 1, 1986·European Journal of Clinical Microbiology·V Hopwood, D W Warnock
Jan 1, 1984·Immunology Letters·W Karwowska, B Lisowska-Grospierre
Jun 25, 1993·International Journal of Food Microbiology·W J Middelhoven, S Notermans
Sep 1, 1985·Diagnostic Microbiology and Infectious Disease·J K Au-YoungE Goldstein
Jan 1, 1993·Diagnostic Microbiology and Infectious Disease·M A PfallerM Densel
Feb 1, 1985·Medit︠s︡inskai︠a︡ sestra·G M Leshchenko
Dec 1, 1987·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·E V WilsonD W Mackenzie
Jan 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·R C Matthews
Jul 18, 2014·PLoS Neglected Tropical Diseases·Gregory GegembauerZoilo Pires de Camargo
Jul 1, 1982·Ophthalmology·D W ParkeL O Gentry
Jul 20, 2004·British Journal of Haematology·Lorna A McLintock, Brian L Jones
Jul 28, 1983·The American Journal of Medicine·J K Todd
Nov 3, 2006·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Shigeki FujitaniLewis Low
Sep 1, 1984·Revista Do Instituto De Medicina Tropical De São Paulo·M C RodriguesC da S Lacaz
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·A Tandon
Mar 12, 2003·Medical Mycology·N KondoriI Mattsby-Baltzer
Jan 1, 1989·Mycoses·R Rüchel
Oct 1, 1993·Clinical Microbiology Reviews·E Reiss, C J Morrison
Sep 1, 1988·Journal of Clinical Microbiology·S J McNabbM H Ivey
Aug 1, 1983·Journal of Clinical Microbiology·C L WellsD J Blazevic
Dec 1, 1989·Journal of Clinical Microbiology·M J Mendes-GianniniA Masuda
Feb 1, 1992·Journal of Clinical Microbiology·G Freitas-da-Silva, M C Roque-Barreira
Feb 11, 1998·Clinical Microbiology Reviews·J P MartínezW L Chaffin
Jun 1, 1984·Journal of Clinical Microbiology·L de RepentignyE Reiss
Jan 1, 1990·Clinical Microbiology Reviews·J M Jones
May 1, 1985·Journal of Clinical Microbiology·J W BaileyJ E Bennett

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