Detection of antibodies to platelets and erythrocytes during infection with haemorrhage-causing Trypanosoma vivax in Ayrshire cattle

Veterinary Parasitology
R K Assoku, P R Gardiner

Abstract

Ayrshire cattle, which were infected with a stock of Trypanosoma vivax from Galana, Kenya, which produced haemorrhagic disease, were examined for the presence of antibodies to erythrocytes and platelets. Antibodies to normal erythrocytes and platelets were detected in the plasma of infected animals using the enzyme-linked immunosorbent assay (ELISA). The antibodies were detectable following the first peak of parasitaemia (10-15 days after infection) and antibody activity was maximal 30-35 days after infection. Plasma from cattle, taken 32 days after infection, precipitated radiolabelled proteins from autologous platelets and, less efficiently, from autologous erythrocytes. Fluorescence-activated cell sorter (FACS) assays demonstrated that erythrocytes and platelets from infected cattle bound IgM and IgG in vivo, and that both normal blood cell types could adsorb these antibodies following incubation in plasma from infected animals. Complement (C3) was similarly adsorbed to erythrocytes during infection. Antibodies adsorbed to infected erythrocytes could be eluted and the eluted antibodies bound to normal erythrocytes, as detected by immunofluorescence, but they did not react with the infecting trypanosome. It is hypothesised th...Continue Reading

References

Jan 1, 1977·Transactions of the Royal Society of Tropical Medicine and Hygiene·M MurrayW I McIntyre
Jun 1, 1989·Veterinary Parasitology·P R GardinerM Murray
Aug 1, 1986·Tropical Animal Health and Production·R J Connor, D J Mukangi
Dec 1, 1972·The Journal of Infectious Diseases·T A Hoffman, E A Edwards
May 1, 1974·The Journal of Clinical Investigation·C E DavisA I Braude
Apr 1, 1974·International Journal for Parasitology·P F Boreham, C A Facer
Jun 20, 1970·British Medical Journal·D BorochovitzJ Metz
Aug 1, 1984·Tropical Animal Health and Production·P L RoederR G Pegram
May 1, 1983·Tropical Animal Health and Production·B T WelldeE A Opiyo
Jan 1, 1984·Vox Sanguinis·P G SørensenK G Schmidt
Jan 1, 1981·Annals of the New York Academy of Sciences·B B VargaftigF Wal
Aug 1, 1982·Experimental Parasitology·J M PrestonB T Wellde
May 1, 1981·Tropical Animal Health and Production·G N MwongelaM A Frazil
Nov 3, 2007·European Journal of Anaesthesiology·G Thorning, K Raghavan

❮ Previous
Next ❯

Citations

Jun 1, 1989·Veterinary Parasitology·P R GardinerM Murray
Dec 1, 1992·Veterinary Parasitology·E Katunguka-RwakishayaP H Holmes
Mar 26, 1998·International Journal for Parasitology·K A Taylor
Mar 7, 2001·Veterinary Parasitology·W H Witola, C E Lovelace
Jan 27, 2009·Infection and Immunity·Nicolas Antoine-MoussiauxDaniel Desmecht
Apr 15, 2009·PloS One·Harry A NoyesJan Naessens
Feb 21, 2014·Parasitology·Ilana Conradie Van WykBanie L Penzhorn
Jul 1, 1992·Research in Veterinary Science·E Katunguka-RwakishayaP H Holmes
Feb 1, 1997·Experimental Parasitology·R A MasakeV M Nantulya
Mar 24, 2016·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Otavio Luiz Fidelis JuniorFabiano Antonio Cadioli
Mar 28, 2008·Memórias do Instituto Oswaldo Cruz·Ana Luiza Alves Rosa OsórioSylvio Celso Gonçalves da Costa
May 16, 2019·Open Biology·Sara Silva PereiraLuisa M Figueiredo
Jul 1, 1992·European Journal of Immunology·J Naessens, D J Williams
Jan 1, 1992·Scandinavian Journal of Immunology. Supplement·D J WilliamsF McOdimba
Jan 9, 2018·Experimental Parasitology·Paula Boeira BassiJoely Ferreira Figueiredo Bittar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.