Detection of antibodies to Staphylococcus aureus Toxic Shock Syndrome Toxin-1 using a competitive agglutination inhibition assay

Letters in Applied Microbiology
V Javid KhojastehHa Foster

Abstract

To develop a competitive agglutination inhibition assay (CAIA) for the detection of anti-Toxic Shock Syndrome Toxin-1 (TSST-1) antibody in serum samples using a commercially available reverse passive agglutination assay (RPLA) kit. TSST-1 toxin and sera were incubated together, so that anti-toxin IgG would complex with the toxin. Latex particles sensitized with rabbit IgG anti-TSST-1 were added to test for un-complexed toxin. The sensitivity and specificity of the CAIA assay was determined relative to positive and negative ELISA results. The sensitivity (proportion of positive ELISA sera which tested positive by CAIA) was 66% whilst the specificity (proportion of ELISA negative sera which tested negative by CAIA) was 75%. Seven sera (14%) were negative by ELISA but positive for CAIA and 12 (18.8%) were positive for ELISA but negative for CAIA, suggesting some interference with the assays. Statistical analysis showed no significant difference between the methods in terms of the numbers of individuals testing positive (chi2, P = 0.04). The CAIA assay allowed detection of anti-TSST-1 within 18 h and was simple to read visually. The method is a useful test for individual serum samples and a preliminary investigation for medical scr...Continue Reading

References

Oct 1, 1989·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·G KunstmannG Pulverer
Jan 1, 1989·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·M BulandaP B Heczko
Mar 1, 1986·Journal of Clinical Microbiology·H IgarashiM S Bergdoll
Apr 1, 1985·Burns, Including Thermal Injury·J D FrameA D Wilmshurst
Oct 1, 1985·The Journal of Trauma·B A FarmerP W Smiley
Sep 25, 1999·Burns : Journal of the International Society for Burn Injuries·C ChildsP J Davenport
Dec 10, 1999·Annals of Clinical Biochemistry·C Selby
Apr 7, 2000·Burns : Journal of the International Society for Burn Injuries·V Edwards-JonesC Childs

❮ Previous
Next ❯

Citations

Feb 11, 2011·Current Infectious Disease Reports·Jonathan A SilversidesAndrew J Ferguson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Related Papers

Burns : Journal of the International Society for Burn Injuries
V Edwards-JonesC Childs
American Journal of Rhinology & Allergy
Lobna M el-FikyAhmed M Adly
Allergology International : Official Journal of the Japanese Society of Allergology
Kumiko KatoKomei Ito
© 2022 Meta ULC. All rights reserved