Detection of antibody classes and subpopulations in myasthenia gravis patients using a new nonradioactive enzyme immunoassay

Muscle & Nerve
C GottiF Clementi

Abstract

To investigate the presence of antibodies in myasthenia gravis (MG) patients, we have developed a new reproducible and sensitive enzyme immunoassay (EIA-AChR), in which a beta subunit-specific monoclonal antibody (mAb 73) immobilizes fetal calf acetylcholine receptors (AChRs). We tested 92 MG patients (42 with positive and 50 with negative antibody titers), 60 healthy controls, and 40 controls with other autoimmune diseases. EIA-AChR detected immunoglobulin G (IgG) titers in all of the seropositive samples, with a significant correlation between these and those obtained using the traditional immunoprecipitation method. Moreover, 5 seronegative patients at immunoprecipitation assay were positive at EIA-AChR. EIA-AChR was also useful in revealing: (1) a seropositive patient subpopulation with generalized MG who had Abs directed against alpha-Bungarotoxin binding sites; and (2) patients with IgM directed against fetal calf AChR (detected in 13 seronegative and 16 seropositive MG patients, and in 6 of the patients with other autoimmune diseases).

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C B Weinberg, Z W Hall
May 1, 1978·Journal of Neurology, Neurosurgery, and Psychiatry·A K LefvertR Pirskanen
Dec 1, 1987·Annals of Neurology·A J SteckM Seligmann
Apr 1, 1988·Neurology·B C SolivenL P Rowland
Jan 1, 1987·Annals of the New York Academy of Sciences·F M HowardL R Elveback
Dec 1, 1985·Journal of Neurology, Neurosurgery, and Psychiatry·A Vincent, J Newsom-Davis
Apr 1, 1973·Analytical Biochemistry·J Schmidt, M A Raftery
Mar 1, 1984·Neurology·C GottiF Clementi
Aug 1, 1994·The Journal of Clinical Investigation·B Vernet-der GarabedianJ F Bach
Jun 23, 1994·The New England Journal of Medicine·D B Drachman
Jul 1, 1993·Immunology Today·M P ProttiB M Conti-Tronconi

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Citations

Apr 13, 2010·Autoimmunity·M Isabel LeiteAngela Vincent
Nov 1, 2003·Annals of the New York Academy of Sciences·Angela VincentWerner Hoch
May 25, 2013·Journal of Clinical Neuromuscular Disease·G Z Huang, Y L Lo

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