PMID: 2491650Jan 1, 1989Paper

Detection of antimitochondrial antibodies: characterization by enzyme immunoassay and immunoblotting

Autoimmunity
N ZurgilY Shoenfeld

Abstract

Mitochondrial antigens were purified from rat liver and characterized by immunoblotting. Sera from 19 well defined patients with primary biliary cirrhosis (PBC) reacted with two mitochondrial polypeptides of 68 Kd and 45 Kd. Antibodies to these antigens were not detected in any of the sera of patients with cirrhosis of the liver, chronic active hepatitis or other autoimmune diseases. The two polypeptides were derived from the soluble fraction of the mitochondrial matrix. An enzyme-linked immunosorbent assay (ELISA) employing these rat liver mitochondrial antigens is described. Positive results were obtained with all except one PBC sera (95%), five out of 47 patients with cirrhosis (11%), one out of 20 patients with chronic active hepatitis (5%), and two out of 19 patients with various autoimmune disorders (11%). The titers detected in PBC were markedly higher than those recorded in patients with other liver and autoimmune diseases. Strong correlation was found between immunoblotting and the ELISA in determining antimitochondrial antibodies. The ELISA presented is easily performed and seems to be a useful diagnostic tool for antimitochondrial antibodies in patients with PBC.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J Lindenborn-FotinosP A Berg
Sep 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M MannsK H Meyer Zum Büschenfelde
Jan 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M E GershwinI R Mackay
Jan 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P A Berg, R Klein
Mar 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C D SurhM E Gershwin
Feb 26, 1987·The New England Journal of Medicine·M M Kaplan
Jan 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H IshiiT Namihisa
Jul 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M M KaplanE G Quaroni

❮ Previous
Next ❯

Citations

May 1, 1992·Journal of Clinical Immunology·N ZurgilC Luderschmidt
Sep 1, 1991·Journal of Clinical Immunology·N ZurgilY Shoenfeld
Dec 9, 1998·Immunologic Research·Y Bar-DayanY Shoenfeld
Jan 1, 1992·Immunologic Research·M SchlesingerY Shoenfeld
Apr 18, 2013·Immunologic Research·Dimitrios P BogdanosYehuda Shoenfeld
Jul 12, 2001·Journal of Gastroenterology and Hepatology·M ShibataK Mitamura
Mar 21, 1998·Journal of Gastroenterology and Hepatology·M NishiokaM P Manns
Aug 17, 2006·Journal of Gastroenterology and Hepatology·Saeko NezuShin-Ichi Takahashi
Dec 26, 2012·Autoimmunity Reviews·Dimitrios P BogdanosYehuda Shoenfeld
Feb 1, 1995·Digestive Diseases and Sciences·A J Czaja

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.