Detection of antitumor promoting activity in Raji cells carrying Epstein-Barr virus genome by immunoblotting analysis

Bioscience, Biotechnology, and Biochemistry
A KondoK Okazaki

Abstract

Extract of Raji cells treated with sodium n-butyrate (1 mM) and a tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA, 40 ng/ml), was analyzed by immunoblotting using ten human sera with different antibody titers against Epstein-Barr virus early antigens. Two human sera reacted with one induced polypeptide of 48 kDa and its induction was inhibited by curcumin (4 micrograms/ml), an antitumor promoter from turmeric. A mouse antiserum against P3HR-1 cells treated with TPA and sodium n-butyrate also detected the 48-kDa polypeptide in Raji cells treated with TPA at concentrations of 2.5 to 80 ng/ml. These results indicate that the immunoblotting analysis can be used in a confirmation test for detection of antitumor promoting activity.

References

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Citations

May 4, 2011·Reviews in Medical Virology·Michael NevelsChristina Paulus
Apr 24, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yih Yih KokAbdul Manaf Ali

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