PMID: 9419787Oct 1, 1994Paper

Detection of apoptosis in human and rat ovarian follicles

Journal of the Society for Gynecologic Investigation
G N PiquetteM L Polan

Abstract

The purpose of this study was to determine whether cells acquired from individual human preovulatory follicles undergo apoptosis (physiologic cell death) and, if so, to correlate the degree of apoptosis with characteristics of the follicles or the oocytes derived from the follicles. We devised a sensitive nonradioactive method for detecting apoptotic DNA fragmentation in small numbers of cells derived from rat atretic follicles and follicular aspirates of patients undergoing assisted reproductive technologies. Using this method, apoptotic DNA was detected in rat atretic follicles, with optimal detection at 10-100 ng. Furthermore, apoptotic DNA was detected in some, but not all individual human follicular aspirates from several patients, and was found in follicles that produced oocytes that fertilized and developed into embryos. Apoptosis occurs in cells from human ovarian preovulatory follicles and may be a normal physiologic process of the follicle during luteinization.

References

Dec 16, 1991·Biochemical and Biophysical Research Communications·C GiannakisP D Zalewski
Dec 1, 1987·The Journal of Pathology·A H Wyllie
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie

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Citations

Oct 22, 2008·Journal of Assisted Reproduction and Genetics·Pericles Assad Hassun FilhoEduardo Leme Alves da Motta
Mar 7, 2009·Biology of Reproduction·Candace M TingenTeresa K Woodruff
Jan 15, 1997·Transplantation·J LaineL Dunkel
May 1, 1996·Obstetrical & Gynecological Survey·S J SpencerR B Jaffe
Oct 12, 2001·Annals of the New York Academy of Sciences·C SimónA Pellicer
Jul 10, 2001·The Journal of Clinical Endocrinology and Metabolism·T E VaskivuoJ S Tapanainen
Mar 29, 2007·Reproduction, Fertility, and Development·Qiang Wang, Qing-Yuan Sun
Jul 16, 2008·Apoptosis : an International Journal on Programmed Cell Death·Dmitri V KryskoKatharina D'Herde
Oct 12, 2000·Baillière's Best Practice & Research. Clinical Obstetrics & Gynaecology·C SimónA Pellicer

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis